Surface plasmonic gold nanorods for enhanced two-photon microscopic imaging and apoptosis induction of cancer cells
Surface plasmonic gold nanorods for enhanced two-photon microscopic imaging and apoptosis induction of cancer cells
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DOI：
10.1016/j.biomaterials.2010.08.068
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发表时间：
2010-12-01
期刊：
影响因子：
14
通讯作者：
Gu, Min
中科院分区：
文献类型：
--
作者：
Li, Jing-Liang;Gu, Min
由飞秒激光器驱动的双光子显微镜是用于癌症发光成像和局部显微手术的有前景的工具，但是破坏细胞所需的高能量限制了其医疗应用在这项工作中，金纳米棒与转铁蛋白结合，用于有效靶向双光子发光成像和增强癌细胞的显微手术由于金纳米棒的双光子激发截面大，金纳米棒的效率比荧光素高一百倍异硫氰酸盐（FITC）是癌细胞三维成像中常用的分子染料 金纳米棒增强的光吸收和能量转换，使治疗细胞的能量注量比没有金纳米棒时低两个数量级 通过操纵能量注量，实现了癌细胞的凋亡 在相同功率密度下，诱导细胞凋亡的能量注量小于坏死细胞的 20% 金纳米棒增强发光成像与癌症细胞凋亡诱导相结合cells 为癌症诊断和治疗提供了医疗安全的基于飞秒激光的成像和显微手术系统 (C) 2010 Elsevier Ltd 保留所有权利
Two-photon microscopy powered by a femtosecond laser is a promising tool for luminescence imaging and localized microsurgery of cancers However the high energy required to destruct cells limits its medical applications In this work gold nanorods were conjugated with transferrin for efficient targeting two-photon luminescence imaging and enhanced microsurgery of cancer cells Due to the large two-photon excitation cross section of gold nanorods gold nanorods are a hundred times more efficient than Fluorescein isothiocyanate (FITC) a common molecular dye in three-dimensional imaging of cancer cells The enhanced light absorption and energy conversion by gold nanorods enable treatment of cells with energy fluences two orders of magnitude below that in the absence of gold nanorods By manipulating the energy fluence apoptosis of cancer cells has been achieved At a same power density the energy fluence for apoptosis induction is less than 20% of that for necrosis Gold nanorods-enhanced luminescence imaging coupled with apoptosis induction of cancer cells provides a medically safe femtosecond laser-based imaging and microsurgery system for cancer diagnosis and treatment (C) 2010 Elsevier Ltd All rights reserved