Effects of alternate-day fasting on high-fat diet-induced insulin resistance in rat skeletal muscle
Effects of alternate-day fasting on high-fat diet-induced insulin resistance in rat skeletal muscle
复制标题
DOI：
10.1016/j.lfs.2013.06.007
复制
发表时间：
2013-08-14
期刊：
影响因子：
6.1
通讯作者：
Terada, Shin
中科院分区：
文献类型：
--
作者：
Higashida, Kazuhiko;Fujimoto, Eri;Terada, Shin
目标：本研究的目的是探讨隔日禁食（ADF）对高脂饮食大鼠骨骼肌胰岛素刺激的葡萄糖转运活性的影响。主要方法：雄性Wistar大鼠分为高脂饮食组（n = 24）和普通饮食组（Chow，n = 12），共10周。4周后将喂食高脂饲料的大鼠分为两组。一组在随后的6周内接受ADF（HF-ADF，n = 12），另一组维持自由采食饮食（HF-AL，n = 12）。10周的饮食干预后，胰岛素刺激的葡萄糖摄取和胰岛素耐量试验（ITT）的测量performed.Key发现：而在HF-AL组的总腹内脂肪量显着高于在周和HF-ADF组，有没有周和HF-ADF组之间的显着差异。然而，胰岛素刺激的骨骼肌中的葡萄糖摄取在两个高脂肪喂养组中均显著低于普通饲料组。HF-AL组大鼠肌肉GLUT-4蛋白含量显著低于普通饲料组（接近30%），HF-ADF组大鼠肌肉GLUT-4蛋白含量进一步降低（接近42%）。HF-ADF和HF-AL组大鼠在ITT.Significance期间的胰岛素抵抗降低程度低于Chow组大鼠：ADF无法消除高脂饮食诱导的肌肉胰岛素抵抗，尽管总腹内脂肪量大幅减少。这可能是由于与饲料组相比，HF-AL和HF-ADF大鼠中的GLUT-4蛋白减少所致。(C)2013 Elsevier Inc. All rights reserved.
Aims: The purpose of this study was to investigate the effects of alternate-day fasting (ADF) on insulin-stimulated glucose transport activity in skeletal muscle in rats fed a high-fat diet.Main methods: Male Wistar rats were placed on a high-fat diet (n = 24) or standard chow diet (Chow, n = 12) for 10 weeks. Rats fed the high-fat diet were separated into two groups after 4 weeks. One group was subjected to ADF for the subsequent 6 weeks (HF-ADF, n = 12), and the other group was maintained on an ad libitum diet (HF-AL, n = 12). After the 10-week dietary intervention, measurements of insulin-stimulated glucose uptake and insulin tolerance test (ITT) were performed.Key findings: Whereas the total intra-abdominal fat mass in the HF-AL group was significantly higher than in the Chow and HF-ADF groups, there was no significant difference between the Chow and HF-ADF groups. However, insulin-stimulated glucose uptake in skeletal muscles was significantly lower in both high-fat fed groups than in the Chow group. Muscle GLUT-4 protein content in HF-AL is significantly lower (similar to 30%) than in Chow, and further reduction (similar to 42%) was observed in the HF-ADF group rats. The HF-ADF and HF-AL group rats had less reduction in glycemia than did the Chow group rats during ITT.Significance: ADF was unable to eliminate high-fat diet-induced muscle insulin resistance, despite a substantial decrease in total intra-abdominal fat mass. This might have resulted from a reduction in GLUT-4 protein in both HF-AL and HF-ADF rats compared to the Chow group. (C) 2013 Elsevier Inc. All rights reserved.