THE INVIVO FORMATION OF CITRATE INDUCED BY FLUOROACETATE AND ITS SIGNIFICANCE
THE INVIVO FORMATION OF CITRATE INDUCED BY FLUOROACETATE AND ITS SIGNIFICANCE
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DOI：
10.1113/jphysiol.1949.sp004456
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发表时间：
1949-01-01
影响因子：
5.5
通讯作者：
PETERS, RA
中科院分区：
文献类型：
--
作者：
BUFFA, P;PETERS, RA
方法动物。这些大多是体重约200克的白化大鼠，它们被喂食老鼠块；用斑纹大鼠做了一些实验。Sodiumjfuoroacetate。（NaFlAc）是BC Saunders博士（剑桥）好心提供的一种纯样品，氟化物含量低于0-1%。给药。NaFlAc, 5 mg /kg。，溶解于0.5 ml 0.9% NaCl中，腹腔注射；对照组同样注射0.5 ml, 0-9% NaCL。室温保存，1小时后处死。除非另有说明，否则一律斩首。乌头酸酶的制备。一般来说，从鸽子的胸肌和大鼠的心脏制备酶都遵循了Johnson（1939）的方法。迅速从被斩首的动物身上取出组织，切成小块，在用沙子和五份pH 7-4 （0-1M）的磷酸盐缓冲液冷藏的臼中研磨。当com。完全分散后，在冷室以4000 RPM离心8-10分钟。将上清玫瑰色悬浮液滗出，用作“原液酶”。马上是PH. CX。32
METHODS Animals. These were mostly albino rats of about 200 g. weight, whichwere fed upon rat cubes; some experiments were made with piebald rats. Sodiumjfuoroacetate.(NaFlAc) was a pure sample kindly given by Dr BC Saunders (Cambridge), containing less than 0-1% fluoride. Administration of the poison. NaFlAc, 5 mg./kg., was dissolved in 0.5 ml. 0 9% NaCl and injected intraperitoneally; the controls were similarly injected with 0 5 ml. 0-9% NaCL. The animals were kept at room temperature and killed after 1 hr., unless otherwise stated, by decapitation.Preparation of aconitase. In general, the method of Johnson (1939) has been followed both for the preparation of the enzyme from the pectoral muscle of the pigeon and from the heart of the rat. The tissue was quickly removed from the decapitated animal, cut into small pieces and ground in a mortar kept ice cold with sand and five parts of phosphate buffer pH 7-4 (0-1M). When com. pletely dispersed, the mixture was centrifuged for 8-10 min. at 4000 rpm in the cold-room. The supernatant rose-tinted suspension was decanted and used as the'stock enzyme'. Immediately PH. CX. 32