Frequent detection of bocavirus DNA in German children with respiratory tract infections
Frequent detection of bocavirus DNA in German children with respiratory tract infections
复制标题
DOI：
10.1186/1471-2334-6-109
复制
发表时间：
2006-07-11
影响因子：
3.7
通讯作者：
Kreth, Hans Wolfgang
中科院分区：
文献类型：
--
作者：
Weissbrich, Benedikt;Neske, Florian;Kreth, Hans Wolfgang
背景：在相当大比例的疑似感染源的呼吸道疾病中，病因不明。其中一些病例可能是由最近描述的人类博卡病毒（hBoV）引起的。本研究的目的是调查的频率和潜在的临床相关性hBoV在儿科patients.Methods：我们测试了835鼻咽吸出物（NPA）之间获得2002年和2005年从儿科住院患者急性呼吸道疾病在维尔茨堡大学，德国，hBoV DNA的存在。结果：在87例（10.3%）非结核性肺疾病患者中检出HBoVDNA。感染hBoV的婴儿和儿童的中位年龄为1.8岁（平均年龄2.0岁;范围18天-8岁）。hBoV感染全年都有发现，但大多数发生在冬季。在34份（39.1%）hBoV阳性样品中发现了合并感染。RSV、甲型流感病毒和腺病毒最常被检测为共感染因子。NP-1区PCR产物的序列测定显示，不同年份获得的核苷酸序列与瑞典病毒ST 1和ST 2相比具有高度同一性（99%）。hBoV与一个独特的呼吸道表现的关联是不明显的。结论：HBoV是经常被发现在NPA的住院婴儿和儿童急性呼吸道疾病。证明hBoV的临床相关性是具有挑战性的，因为应用科赫的一些修订假设是不可能的。由于hBoV和其他呼吸道病原体的合并感染率很高，hBoV和呼吸道疾病之间的关联尚未得到证实。
Background: In a substantial proportion of respiratory tract diseases of suspected infectious origin, the etiology is unknown. Some of these cases may be caused by the recently described human bocavirus (hBoV). The aim of this study was to investigate the frequency and the potential clinical relevance of hBoV in pediatric patients.Methods: We tested 835 nasopharyngeal aspirates (NPA) obtained between 2002 and 2005 from pediatric in-patients with acute respiratory tract diseases at the University of Wurzburg, Germany, for the presence of hBoV DNA. The specificity of positive PCR reactions was confirmed by sequencing.Results: HBoV DNA was found in 87 (10.3 %) of the NPAs. The median age of the infants and children with hBoV infection was 1.8 years (mean age 2.0 years; range 18 days -8 years). Infections with hBoV were found year-round, though most occurred in the winter months. Coinfections were found in 34 (39.1 %) of the hBoV positive samples. RSV, influenza A, and adenoviruses were most frequently detected as coinfecting agents. Sequence determination of the PCR products in the NP-1 region revealed high identity (99 %) between the nucleotide sequences obtained in different years and in comparison to the Swedish viruses ST1 and ST2. An association of hBoV with a distinct respiratory tract manifestation was not apparent.Conclusion: HBoV is frequently found in NPAs of hospitalized infants and children with acute respiratory tract diseases. Proving the clinical relevance of hBoV is challenging, because application of some of Koch's revised postulates is not possible. Because of the high rate of coinfections with hBoV and other respiratory tract pathogens, an association between hBoV and respiratory tract diseases remains unproven.