Human lung tumor-associated antigen identified as an extracellular matrix adhesion molecule.
Human lung tumor-associated antigen identified as an extracellular matrix adhesion molecule.
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DOI：
10.1084/jem.173.5.1111
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发表时间：
1991-05-01
期刊：
影响因子：
--
通讯作者：
Bankert RB
中科院分区：
文献类型：
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作者：
Chen FA;Repasky EA;Bankert RB
一种估计分子量为 160 kD (gp160) 的单链糖蛋白先前被鉴定为人肺肿瘤相关抗原。此处显示该肿瘤标记物与第二种 130 kD 蛋白质非共价相关。对表面碘化肺肿瘤细胞裂解物的连续免疫沉淀研究表明，这种异二聚体复合物在血清学和结构上与最初在活化的 T 淋巴细胞和血小板上发现的整合素 VLA-2 没有区别。肺肿瘤上表达的 VLA-2 样复合物具有与正常细胞上的 VLA-2 观察到的相似的胶原蛋白和层粘连蛋白的 Mg2+ 依赖性结合特征。 RNA 分析表明，人类肺肿瘤表达的 VLA-2 α 链信息比正常成人肺组织至少多 20 倍。这里提出的结果提出了这样一种可能性：VLA-2的过量产生可能通过调节肿瘤的侵袭和转移潜力而参与人类肺部肿瘤的发病机制。
A single chain glycoprotein with an estimated molecular mass of 160 kD (gp160) was previously identified as a human lung tumor-associated antigen. This tumor marker is shown here to be associated noncovalently with a second 130-kD protein. Sequential immunoprecipitation studies of surface iodinated lung tumor cell lysates reveal that this heterodimeric complex is indistinguishable serologically and structurally from the integrin VLA-2, found originally on activated T lymphocytes and platelets. The VLA-2-like complex expressed on the lung tumors possesses similar characteristic Mg2+ dependent binding of collagen and laminin as observed with VLA-2 on normal cells. RNA analysis indicates that human lung tumors express at least 20 times more VLA-2 alpha chain message than normal adult human lung tissue. The results presented here raise the possibility that the overproduction of VLA-2 may be involved in the pathogenesis of human lung tumors by modulating the invasive and metastatic potential of the tumor.