Integrated image and X-ray microanalysis of hepatic lysosomes in a patient with idiopathic hemosiderosis before and after treatment by phlebotomy.
Integrated image and X-ray microanalysis of hepatic lysosomes in a patient with idiopathic hemosiderosis before and after treatment by phlebotomy.
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特发性含铁血黄素沉着症患者放血治疗前后肝溶酶体的综合图像和 X 射线微量分析。
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
D. Wc
中科院分区：
文献类型：
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作者：
Cleton Mi;Roelofs Jm;B. Cj;D. Wc
形态计量学和X-射线元素信息提取的扫描透射电子显微镜（STEM）图像的肝溶酶体的特发性含铁血黄素沉着症患者治疗前和治疗后的放血。感兴趣的元素是铁，以病理学量储存在肝溶酶体结构和铈，用作细胞化学反应后的捕获离子，以检测溶酶体结构中的酸性磷酸酶活性。形态学上，溶酶体结构是异形的，元素铁和铈是不均匀分布的。在16 X 16像素点（整合图像和X射线显微分析）下进行“缩小光栅”（=缩小扫描面积）分析，可以证明处理前后溶酶体横截面结构面积的显著差异。同时，可以观察到放血前后元素铁和铈的相对取向的差异。Chelex离子交换珠，负载有11.5%w/w的铁，并与组织块共包埋，被用作内标。获得平均铁峰与背景的比率，并计算转换为绝对铁浓度的比率的因子。对于肝溶酶体结构，遵循相同的计算程序（现在是每个像素点）。通过放血治疗前后观察到个体溶酶体结构中铁浓度的显著差异。
Morphometrical and X-ray elemental information was extracted from Scanning Transmission Electron Microscopy (STEM) images of hepatic lysosomes of a patient with idiopathic hemosiderosis before and after treatment by phlebotomy. The elements of interest were iron, stored in pathological quantities in hepatic lysosomal structures and cerium, used as a capture ion after a cytochemical reaction to detect acid phosphatase activity in the lysosomal structures. Morphologically the lysosomal structures are heteromorph and the elements iron and cerium are heterogeneously distributed. With "reduced raster" (= reduced scanning area) analysis at 16 X 16 pixelpoints (integrating image and X-ray microanalysis), a marked difference in the area of the cross sectioned lysosomal structures before and after treatment could be demonstrated. Simultaneously the difference in the relative orientation of the elements iron and cerium before and after phlebotomy could be visualized. Chelex ion exchange beads, loaded with 11.5% w/w iron, and coembedded with the tissue blocks, were used as an internal standard. A mean iron peak to background ratio was obtained and a factor, converting ratio to absolute iron concentration, was calculated. The same calculation procedure, now per pixelpoint, was followed for the hepatic lysosomal structures. A marked difference in iron concentration in the individual lysosomal structures was observed before and after treatment by phlebotomy.