ULTRASTRUCTURE AND CYTOCHEMISTRY OF SHELL MEMBRANE-SECRETING REGION OF JAPANESE QUAIL OVIDUCT
ULTRASTRUCTURE AND CYTOCHEMISTRY OF SHELL MEMBRANE-SECRETING REGION OF JAPANESE QUAIL OVIDUCT
复制标题
DOI：
10.1002/aja.1001310302
复制
发表时间：
1971-01-01
影响因子：
--
通讯作者：
HOFFER, AP
中科院分区：
文献类型：
--
作者：
HOFFER, AP
通过光学显微镜和电子显微镜以及细胞化学技术检查日本鹌鹑输卵管的峡部粘膜或壳膜分泌部分。对壳膜也作了初步研究，峡部粘膜呈纵行褶皱，外覆由纤毛细胞和粘液分泌细胞组成的单层柱状上皮。许多卷曲的单管腺体密集地排列在表面上皮下，并通过短导管与之相连。管状腺由单细胞型主细胞组成，主细胞内含有大量大的分泌颗粒。成熟的颗粒显示一个偏心放置的致密的球形核心，周围环绕着一个密度较低的皮质。壳膜由在膜平面上随机排列的交叉纤维组成，每根纤维由髓质和皮质组成。细胞化学方法表明，主要的细胞分泌颗粒由碳水化合物和蛋白质的复合物组成。蛋白质富含巯基;多糖部分既不硫酸化也不富含羧基。粘液素颗粒由非硫酸化的含碳水化合物物质组成。壳膜含有碳水化合物和蛋白质两种成分。推测提出了完整的壳膜和它们的分泌前体的各种成分之间的形态关系。
The isthmic mucosa or shell membrane‐secreting portion of the Japanese quail oviduct was examined by light and electron microscopy and by cytochemical techniques. Preliminary studies of the shell membrane were also made.The mucosa of the isthmus is thrown into numerous longitudinal folds which are covered by a simple columnar epithelium consisting of ciliated and mucussecreting cells. Numerous convoluted simple tubular glands lie densely packed beneath the surface epithelium and are connected to it by short ducts. The tubular glands are made up of a single cell type, the principal cell, which contains numerous large secretory granules. Mature granules display an eccentrically placed dense spherical core surrounded by a less dense cortex. The shell membrane is composed of a meshwork of intersecting fibers oriented randomly in the plane of the membrane; each fiber is made up of a medulla and cortex.Cytochemical methods indicate that the principal cell secretory granules consist of a complex of carbohydrate with protein. The protein is rich in sulfhydryl groups; the polysaccharide moiety is neither sulfated nor rich in carboxyl groups. The mucigen granules are composed of a nonsulfated carbohydrate‐containing substance. The shell membrane has both carbohydrate and protein components. Speculations are presented on the morphological relationships between the various components of the completed shell membrane and their secretory precursors.