Contrasting environmental factors drive bacterial and eukaryotic community successions in freshly deglaciated soils
Contrasting environmental factors drive bacterial and eukaryotic community successions in freshly deglaciated soils
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对比环境因素驱动新消融土壤中的细菌和真核生物群落演替
DOI：
10.1093/femsle/fnz229
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发表时间：
2019
影响因子：
2.1
通讯作者：
Shichang Kang
中科院分区：
文献类型：
--
作者：
Ajmal Khan;Weidong Kong;Said Muhammad;Fei Wang;Guoshuai Zhang;Shichang Kang
冰川退缩使冰川消退的土壤暴露于微生物的定植和演替;然而，细菌和真核生物演替驱动因素的差异在很大程度上仍然难以捉摸。利用qPCR、末端限制性片段长度多态性（T-RFLP）和克隆文库测序技术，研究了青藏高原土壤细菌和真核生物在冰川消融期（10 a）的定植和群落沿着的演替规律。结果表明，细菌和真核生物在冰川消退的第一年迅速定居在土壤中，此后缓慢增加，分别从107增加到1010和1011个基因拷贝g-1土壤。细菌和真核生物群落的变化分为不同的阶段，包括早期（0-2岁），过渡期（3-5岁）和晚期（6-10岁）。土壤因子对细菌群落演替的影响占主导地位（47.7%），其中土壤水分对细菌群落演替的贡献率为26.9%。与此相反，真核生物群落的演替主要是由冰消年龄（22.2%）。优势菌类群为蓝细菌，从早期到过渡期迅速减少。早期土壤中真核生物以冰川沉积的尾藻为主，晚期土壤中绿色藻类绿藻明显增多。我们的研究结果揭示了驱动细菌和真核生物群落演替的对比环境因素。
Glacier retreats expose deglaciated soils to microbial colonization and succession; however, the differences in drivers of bacterial and eukaryotic succession remain largely elusive. We explored soil bacterial and eukaryotic colonization and yearly community succession along a deglaciation chronosequence (10 years) on the Tibetan Plateau using qPCR, terminal restriction fragment length polymorphism (T-RFLP) and sequencing of clone libraries. The results exhibited that bacteria and eukaryotes rapidly colonized the soils in the first year of deglaciation, thereafter slowly increasing from 107up to 1010and 1011gene copies g−1soil, respectively. Bacterial and eukaryotic community changes were observed to group into distinct stages, including early (0–2 year old), transition (3–5 year old) and late stages (6–10 year old). Bacterial community succession was dominantly driven by soil factors (47.7%), among which soil moisture played a key role by explaining 26.9% of the variation. In contrast, eukaryotic community succession was dominantly driven by deglaciation age (22.2%). The dominant bacterial lineage wasCyanobacteria, which rapidly decreased from the early to the transition stage. Eukaryotes were dominated by glacier-originatedCercozoain early stage soils, while green algaeChlorophytasubstantially increased in late stage soils. Our findings revealed contrasting environmental factors driving bacterial and eukaryotic community successions.