Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation.
Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation.
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DOI：
10.1038/s41598-017-02327-6
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发表时间：
2017-05-19
影响因子：
4.6
通讯作者：
Wellington EMH
中科院分区：
文献类型：
--
作者：
Lidbury IDEA;Murphy ARJ;Fraser TD;Bending GD;Jones AME;Moore JD;Goodall A;Tibbett M;Hammond JP;Scanlan DJ;Wellington EMH
在土壤中，磷（P）以多种有机和无机形式存在。然而，植物只能获得无机正磷酸盐（Pi），这意味着全球作物产量经常受到磷可用性的限制。为了克服这个问题，大量使用磷酸盐肥料，往往带来负面的环境和社会经济后果。土壤中的有机磷部分含有磷脂，磷脂迅速降解，导致生物有效磷的释放。然而，这一过程背后的机制仍然未知。我们鉴定并实验证实了两种分泌型甘油磷酸二酯酶GlpQI和GlpQII的功能，它们分别存在于施氏假单胞菌DSM 4166和荧光假单胞菌SBW 25中。一系列共培养实验表明，在这些假单胞菌菌株中，甘油磷酸胆碱及其分解产物G3 P的裂解发生在细胞外，使其他细菌受益于这种代谢。宏基因组和元转录组数据集的分析表明，这种特性在土壤细菌中广泛存在，放线菌和变形菌，特别是β-变形菌和γ-变形菌，可能是主要的参与者。
In soils, phosphorus (P) exists in numerous organic and inorganic forms. However, plants can only acquire inorganic orthophosphate (Pi), meaning global crop production is frequently limited by P availability. To overcome this problem, rock phosphate fertilisers are heavily applied, often with negative environmental and socio-economic consequences. The organic P fraction of soil contains phospholipids that are rapidly degraded resulting in the release of bioavailable Pi. However, the mechanisms behind this process remain unknown. We identified and experimentally confirmed the function of two secreted glycerolphosphodiesterases, GlpQI and GlpQII, found in Pseudomonas stutzeri DSM4166 and Pseudomonas fluorescens SBW25, respectively. A series of co-cultivation experiments revealed that in these Pseudomonas strains, cleavage of glycerolphosphorylcholine and its breakdown product G3P occurs extracellularly allowing other bacteria to benefit from this metabolism. Analyses of metagenomic and metatranscriptomic datasets revealed that this trait is widespread among soil bacteria with Actinobacteria and Proteobacteria, specifically Betaproteobacteria and Gammaproteobacteria, the likely major players.