Prestructured MALDI-MS sample supports
Prestructured MALDI-MS sample supports
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DOI：
10.1021/ac000092a
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发表时间：
2000-08-01
影响因子：
7.4
通讯作者：
Nordhoff, E
中科院分区：
文献类型：
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作者：
Schuerenbeg, M;Luebbert, C;Nordhoff, E
已经开发了预结构化MALDI-MS样品支持物，其简化了生物分子的高通量分析并提高了检测灵敏度。质谱样品支持物上涂有一薄层疏水的Tenon，Tenon上有一排200 μ m的金斑点，这些斑点提供了亲水的样品锚。每个转移的样品液滴接触一个锚，在溶剂蒸发后，由于Tenon表面的强烈防水性质，样品仅沉积在其顶部。初始基质浓度保持较低，使得样品在结晶开始前浓缩超过2个数量级。结果，如从100阿莫尔各种肽、1 fmol DNA 20 mer和5 fmol 130 bp PCR产物记录的质谱所证明的，检测灵敏度提高。为了MALDI-MS性能（样品点尺寸接近激光照射点尺寸）、为了短的分析时间（预定的样品坐标）和为了高通量样品制备（根据1536微量滴定板格式的样品锚阵列），亲水锚的尺寸和间距被优化。
Prestructured MALDI-MS sample supports have been developed that simplify high-throughput analysis of biomolecules and improve the detection sensitivity. The mass spectrometric sample support is coated with a thin layer of hydrophobic Tenon that carries an array of 200-mu m gold spots, which provide hydrophilic sample anchors. Each transferred sample droplet contacts one anchor, on top of which, after solvent evaporation, the sample is exclusively deposited due to the strongly water repellent nature of the Tenon surface. The initial matrix concentration is kept low, enabling sample up-concentration by more than 2 orders of magnitudes before crystallization commences. As a result, the detection sensitivity is improved as documented by mass spectra recorded from 100 amol of various peptides, 1 fmol of a DNA 20 mer, and 5 fmol of a 130 bp PCR product. Size and spacing of the hydrophilic anchors are optimized for MALDI-MS performance (sample spot size approximate to laser irradiation spot size), for short analysis times (predetermined sample coordinates), and for high throughput sample preparation (sample anchor array according to the 1536 microtiter plate format).