Changes in Ultrastructure and Cytoskeletal Aspects of Human Normal and Osteoarthritic Chondrocytes Exposed to Interleukin-1β and Cyclical Hydrostatic Pressure.
Changes in Ultrastructure and Cytoskeletal Aspects of Human Normal and Osteoarthritic Chondrocytes Exposed to Interleukin-1β and Cyclical Hydrostatic Pressure.
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DOI：
10.3390/ijms161125936
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发表时间：
2015-10-30
影响因子：
5.6
通讯作者：
Fioravanti A
中科院分区：
文献类型：
--
作者：
Pascarelli NA;Collodel G;Moretti E;Cheleschi S;Fioravanti A
本研究的目的是检查暴露于白细胞介素-1β (IL-1β) 和循环静水压 (HP) 的人类正常软骨细胞和骨关节炎 (OA) 软骨细胞的超微结构和细胞骨架组织。通过透射电子显微镜 (TEM) 和扫描电子显微镜 (SEM) 进行的形态学检查揭示了正常软骨细胞和 OA 软骨细胞在核和细胞质水平上的差异。 IL-1β (5 ng/mL) 诱导线粒体和高尔基体数量减少，并且富含空泡化和边缘染色质的细胞百分比显着增加。周期性HP（1-5 MPa，0.25 Hz，持续3小时）不会改变正常软骨细胞的形态，但对OA软骨细胞有有益作用，增加细胞器数量。接受IL-1β和HP处理的正常细胞和OA细胞恢复了细胞质超微结构。正常软骨细胞的免疫荧光 (IF) 检查显示肌动蛋白信号在细胞质顶端极化，微管蛋白和波形蛋白均匀分布在整个细胞质中，纽蛋白显示质膜下的间断模式。在 OA 软骨细胞中，这些蛋白质部分失去了组织。 IL-1β 刺激会在两种类型的细胞中引起细胞骨架组织的改变； HP 抵消了 IL-1β 的负面影响。我们的结果显示正常软骨细胞和 OA 软骨细胞在核、细胞质和细胞骨架水平上存在结构差异。 IL-1β 诱导超微结构和细胞骨架的改变，并被周期性的低 HP 所抵消。
The aim of this study was to examine the ultrastructure and cytoskeletal organization in human normal and Osteoarhritic (OA) chondrocytes, exposed to interleukin-1β (IL-1β) and cyclic hydrostatic pressure (HP). Morphological examination by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) revealed differences between normal and OA chondrocytes at the nuclear and cytoplasmic level. IL-1β (5 ng/mL) induced a decrease of the number of mitochondria and Golgi bodies and a significant increase on the percentage of cells rich in vacuolization and in marginated chromatin. Cyclical HP (1–5 MPa, 0.25 Hz, for 3 h) did not change the morphology of normal chondrocytes, but had a beneficial effect on OA chondrocytes increasing the number of organelles. Normal and OA cells subjected to IL-1β and HP recovered cytoplasmic ultrastructure. Immunofluorescence (IF) examination of normal chondrocytes showed an actin signal polarized on the apical sides of the cytoplasm, tubulin and vimentin uniformly distributed throughout cytoplasm and vinculin revealed a punctuated pattern under the plasma membrane. In OA chondrocytes, these proteins partially lost their organization. Stimulation with IL-1β caused, in both type of cells, modification in the cytoskeletal organization; HP counteracted the negative effects of IL-1β. Our results showed structural differences at nuclear, cytoplasmic and cytoskeletal level between normal and OA chondrocytes. IL-1β induced ultrastructural and cytoskeletal modifications, counteracted by a cyclical low HP.