Rapid biochemical test to identify verocytotoxin-positive strains of Escherichia coli serotype O157
Rapid biochemical test to identify verocytotoxin-positive strains of Escherichia coli serotype O157
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快速生化检测鉴定 O157 血清型大肠杆菌 Verocytotoxin 阳性菌株
DOI：
10.1128/jcm.28.10.2165-2168.1990
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发表时间：
1990
影响因子：
9.4
通讯作者：
A. Borczyk
中科院分区：
文献类型：
--
作者：
J. S. Thompson;D. Hodge;A. Borczyk
以4-甲基伞形花环-β-D-葡萄糖醛酸苷(MUG)为底物，采用荧光法鉴定大肠杆菌。大多数菌株产生β-葡萄糖苷酸酶，因此MUG阳性。建立了一种20分钟的程序来检测从临床标本中分离的23个属的1,295株细菌的葡萄糖醛酸苷酶活性。除大肠杆菌外，很少有其他生物呈MUG阳性。分离的682株大肠埃希菌中，MUG阳性630株(92.4%)。对188株O157型大肠埃希菌进行检测，155株O157：H7、10株O157：H-和1株O157：H(粗)大肠埃希菌MUG阴性。166株细菌培养均呈虫胞毒素阳性。在其余22株O157菌株中，2株为O157：H-，1株为O157：H(粗)，19株为其他H型(H6、H16、H19、H25、H42、H45)，均为MUG阳性。22种细菌培养均为虫卵毒素阴性。快速MUG程序可以用来预测Vc毒素阳性的E.ColiO157菌株；也就是说，MUG阴性的E.ColiO157菌株很有可能是Vc毒素阳性的。
Fluorogenic procedures were used with the substrate 4-methylumbelliferyl-beta-D-glucuronide (MUG) to identify Escherichia coli. Most strains produced beta-glucuronidase and, thus, were MUG positive. A 20-min procedure was developed to detect glucuronidase activity in 1,295 bacterial cultures, representing 23 genera, of strains that were isolated from clinical specimens. Very few organisms other than E. coli were MUG positive. Of 682 E. coli strains that were isolated, 630 (92.4%) were MUG positive. When an additional 188 E. coli serotype O157 isolates were examined, 155 E. coli O157:H7, 10 E. coli O157:H-, and 1 E. coli O157:H (rough) isolate were MUG negative. All 166 cultures were verocytotoxin positive. Of the remaining 22 E. coli O157 isolates, 2 isolates were O157:H-, 1 isolate was O157:H (rough), and 19 isolates were other H types (H6, H16, H19, H25, H42, and H45); these 22 isolates were MUG positive. All 22 cultures were verocytotoxin negative. The rapid MUG procedure can be used to predict verocytotoxin-positive isolates of E. coli O157; that is, there is a very good likelihood that MUG-negative E. coli O157 isolates are verocytotoxin positive.