Identification and characterization of differentially expressed exosomal microRNAs in bovine milk infected with Staphylococcus aureus
Identification and characterization of differentially expressed exosomal microRNAs in bovine milk infected with Staphylococcus aureus
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DOI：
10.1186/s12864-019-6338-1
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发表时间：
2019-12-05
期刊：
影响因子：
4.4
通讯作者：
Zhao, Xin
中科院分区：
文献类型：
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作者：
Ma, Shaoyang;Tong, Chao;Zhao, Xin
背景：乳源性exosome中的microRNAs（miRNAs）可能反映乳腺炎引起的病理生理变化。本研究分析了金黄色葡萄球菌（Staphylococcus aureus，S. aureus)具有良好的抗菌活性。结果：从健康奶牛（n = 3，对照组）和健康奶牛（n = 3，对照组）的乳汁exosomes中提取总RNA，用RT-PCR方法检测了差异表达miRNA的靶基因，并对bta-miR-378和bta-miR-185的靶基因进行了验证。金黄色葡萄球菌感染奶牛（n = 6，SA组）。共鉴定出290个miRNA，其中221个为已知miRNA，69个为新miRNA。其中22个已知miRNA和15个新的miRNA表达存在差异。DE miRNAs的靶基因在胞内蛋白转运、内质网和同源蛋白结合等方面显著富集。两种miRNA（bta-miR-378和bta-miR-185）的表达具有高的读取计数和log（2）倍数变化（> 3.5），在感染链球菌的乳腺炎乳汁中显著更高。金黄色。结果：在正常人和沙门氏菌exosomes中检测到1个靶基因（VAT 1 L）和5个靶基因（DYRK 1B、MLLT 3、HP 1BP 3、NPR 2和PGM 1）的DE miRNA。鉴定了金黄色葡萄球菌感染的牛奶。进一步验证了两种DE miRNA（bta-miR-378和bta-miR-185）的预测靶点。这些靶基因与疾病的相关性提示，从牛奶安全性的角度出发，我们应特别关注乳腺炎乳中的外切体miRNA。
Background: MicroRNAs (miRNAs) in milk-derived exosomes may reflect pathophysiological changes caused by mastitis. This study profiled miRNAs in exosomes from both normal milk and mastitic milk infected by Staphylococcus aureus (S. aureus). The potential targets for differentially expressed (DE) miRNAs were predicted and the target genes for bta-miR-378 and bta-miR-185 were also validated.Results: Total RNA from milk exosomes was collected from healthy cows (n = 3, the control group) and S. aureus infected cows (n = 6, the SA group). Two hundred ninety miRNAs (221 known and 69 novel ones) were identified. Among them, 22 known and 15 novel miRNAs were differentially expressed. Target genes of DE miRNAs were significantly enriched in intracellular protein transport, endoplasmic reticulum and identical protein binding. The expression of two miRNAs (bta-miR-378 and bta-miR-185) with high read counts and log(2) fold changes (> 3.5) was significantly higher in mastitic milk infected with S. aureus. One target gene (VAT1L) of bta-miR-378 and five target genes (DYRK1B, MLLT3, HP1BP3, NPR2 and PGM1) of bta-miR-185 were validated.Conclusion: DE miRNAs in exosomes from normal and S. aureus infected milk were identified. The predicted targets for two DE miRNAs (bta-miR-378 and bta-miR-185) were further validated. The linkage between the validated target genes and diseases suggested that we should pay particular attention to exosome miRNAs from mastitic milk in terms of milk safety.