Identifying Similar Allergens and Potentially Cross-Reacting Areas Using Structural Database of Allergenic Proteins (SDAP) Tools and D-Graph.
Identifying Similar Allergens and Potentially Cross-Reacting Areas Using Structural Database of Allergenic Proteins (SDAP) Tools and D-Graph.
复制标题
使用过敏蛋白结构数据库 (SDAP) 工具和 D-Graph 识别相似的过敏原和潜在的交叉反应区域。
DOI：
10.1007/978-1-0716-3453-0_18
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Schein,CatherineH
中科院分区：
文献类型：
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作者：
Schein,CatherineH
过敏性蛋白质结构数据库（SDAP）提供了快速搜索工具，以确定过敏原之间的相似性，其IgE表位，并确定任何新蛋白质的潜在过敏性。许多实验室已经使用斑点或微阵列鉴定了IgE结合蛋白及其抗体结合或T细胞表位。本章介绍如何使用SDAP中实现的工具确定这些蛋白质和肽与已知过敏原的关系。人们还可以使用SDAP中实现的这些较小的肽相似性搜索工具进行搜索，以在超过1500个过敏原序列中找到具有低属性距离（PD）值的相似序列。可以通过在SDAP中几乎所有过敏原的蛋白质结构表面上作图来比较序列。一旦用户有了相似序列的唯一列表，就可以根据D图程序自动计算的肽间PD值以2D方式绘制它们。本章从一种类似于Ole e 1（橄榄花粉）过敏原家族的蛋白质开始，一步一步地描述了如何做到这一点。
The Structural Database of Allergenic Proteins (SDAP) provides rapid search tools to identify similarities among allergens, their IgE epitopes, and to determine the potential allergenicity of any novel protein. Many labs have identified IgE-binding proteins and their antibody binding or T cell epitopes using dotspots or microarrays. This chapter describes how to determine the relationship of these proteins and peptides to known allergens using the tools implemented in SDAP. One can also search with these smaller peptide similarity search tool implemented in SDAP to find similar sequences with low property distance (PD) values in the over 1500 sequences of allergens. The sequences can be compared by mapping on the surface of the protein structures provided for nearly all the allergens in SDAP. Once the user has a unique list of similar sequences, they can be graphed in 2D according to interpeptide PD values calculated automatically by the D-graph program. This chapter provides a step-by-step description of how to do this, starting from a protein similar to the Ole e 1 (olive pollen) allergen family.