Immobilized lipase on porous ceramic monoliths for the production of sugar-derived oil gelling agent
Immobilized lipase on porous ceramic monoliths for the production of sugar-derived oil gelling agent
复制标题
将脂肪酶固定在多孔陶瓷整体上用于生产糖基油胶凝剂
DOI：
10.1039/c5ra10570f
复制
发表时间：
2015-01-01
期刊：
影响因子：
3.9
通讯作者：
Zhou, Liya
中科院分区：
文献类型：
--
作者：
Gao, Jing;Feng, Kai;Zhou, Liya
在这项研究中，脂肪酶从假丝酵母99-125固定化戊二醛活化APTES功能化或环氧功能化的多孔陶瓷整体。研究了固定化脂肪酶的活性和稳定性。结果表明，固定化脂肪酶的稳定性较天然脂肪酶有明显提高。以甘露醇和脂肪酸乙烯基酯为底物，将固定化脂肪酶作为催化剂用于生产油凝剂。以Man-8为例，在固定化脂肪酶催化下，反应48 h后，该油凝剂的产率可达78%以上。第5批反应后，收率可保持在50%以上。成胶试验结果表明，该胶凝剂对各种烃类和原油都有较好的胶凝效果。当油胶凝剂用于从两相混合物中回收柴油时，可以回收超过90%的初始柴油。
In this study, lipase from Candida sp. 99-125 was immobilized on glutaraldehyde-activated APTES-functionalized or epoxy-functionalized porous ceramic monoliths. The activity and stability of the immobilized lipases were investigated. The results indicated that the stabilities of immobilized lipases were improved significantly compared to native lipase. The immobilized lipases were used as catalysts for the production of oil gelling agent by using mannitol and fatty acid vinyl ester as substrates. Take Man-8 as an example, the yield of this oil gelling agent was more than 78% after 48 h reaction that catalyzed by the immobilized lipases. After the 5th batch reaction, more than 50% of yield can be maintained. Furthermore, the results of gelation tests indicated that the oil gelling agent can gel various hydrocarbons and oils. When the oil gelling agent was used to recover diesel from a biphasic mixture, more than 90% of the initial diesel can be recovered.