Effects of host interaction with Wolbachia on cytoplasmic incompatibility in the two-spotted spider mite Tetranychus urticae
Effects of host interaction with Wolbachia on cytoplasmic incompatibility in the two-spotted spider mite Tetranychus urticae
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宿主与沃尔巴克氏体相互作用对二斑叶螨细胞质不相容性的影响
DOI：
10.1111/bij.12804
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发表时间：
2016-09
影响因子：
1.9
通讯作者：
Hong Xiao-Yue
中科院分区：
文献类型：
--
作者：
Sun Jian-Xin;Guo Yan;Zhang Xu;Zhu Wen-Chao;Chen Ya-Ting;Hong Xiao-Yue
内共生细菌Wolbachia pipientis感染广泛的节肢动物，并诱导各种生殖异常，包括细胞质不相容性（CI）。对二斑叶螨的三个种群进行了调查。基于基因测序，它们具有不同的宿主遗传背景，但感染了相同的Wolbachia菌株。我们还研究了CI水平相对于宿主背景，男性年龄，沃尔巴克氏体密度，锚蛋白（ANK）基因表达的二斑叶螨。本研究结果表明：（1）二斑叶螨种群间的感染性差异可能是宿主遗传背景的结果，（2）男性年龄不是决定感染强度的因素，（3）男性体内Wolbachia密度可能是二斑叶螨发生感染的阈值因素，在此之后，其他因素在决定感染强度方面变得重要。（4）宿主可能通过调节ANK基因的表达来调节CI强度。我们的研究结果描述了一种新的类型之间的相互作用沃尔巴克氏体和它的主机，和相互作用对CI的影响。进一步研究沃尔巴克氏体锚蛋白基因产物的功能及其宿主靶点，特别是在宿主生殖操纵方面，也是必要的。
The endosymbiotic bacterium Wolbachia pipientis infects a wide range of arthropods and induces a variety of reproductive anomalies, including cytoplasmic incompatibility (CI). Three populations of the two-spotted spider mite, Tetranychus urticae, were investigated in the present study. Based on gene sequencing, they had different host genetic backgrounds but were infected with the same Wolbachia strain. We also examined the CI level relative to host background, male age, Wolbachia density, and Ankyrin (ANK) gene expression in T. urticae. The results of the present study suggest that: (1) CI differences between populations appear to be a result of host genetic background; (2) male age is not a factor determining intensity of CI; (3) Wolbachia density in males may serve as threshold factor necessary for the CI to occur in T. urticae, after which other factors become important in determining the strength of CI; and (4) hosts may modulate CI intensity through modulation of ANK gene expression in males. Our results describe a new type of interaction between Wolbachia and its hosts, and the effect of the interactions on CI. Further investigations on the functions of Wolbachia ankyrin gene products and their host targets, particularly with respect to host reproductive manipulation, are also imperative.
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影响因子：
15.9
作者：
Al-Khodor S;Price CT;Kalia A;Abu Kwaik Y
通讯作者：
Abu Kwaik Y
影响因子：
5.8
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
4.2
作者：
Pichon S;Bouchon D;Liu C;Chen L;Garrett RA;Grève P
通讯作者：
Grève P
影响因子：
2.2
作者：
Gotoh, Tetsuo;Sugasawa, Jun;Kitashima, Yasuki
通讯作者：
Kitashima, Yasuki
影响因子：
12.3
作者：
Salzberg SL;Dunning Hotopp JC;Delcher AL;Pop M;Smith DR;Eisen MB;Nelson WC
通讯作者：
Nelson WC