Measurement of pH and dissolved oxygen within cell culture media using a hydrogel microarray sensor
Measurement of pH and dissolved oxygen within cell culture media using a hydrogel microarray sensor
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DOI：
10.1016/j.snb.2007.06.027
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发表时间：
2008-01-15
影响因子：
8.4
通讯作者：
Pishko, Michael V.
中科院分区：
文献类型：
--
作者：
Lee, Seungjoon;They, Bennett L.;Pishko, Michael V.
在不引入外源试剂的情况下感测细胞培养基中的分析物在当今的细胞培养系统中证明是困难的。在本文中，一个完全嵌入式的微阵列传感器，已开发沿着与紧凑的荧光成像系统感测氧气和pH值的非侵入性描述。紧凑型检测系统由超亮蓝色二极管光源、耦合光学器件、干涉滤光片和紧凑型防潮CCD相机组成。微阵列传感器是由光反应注塑成型，并包含两个独立的传感元件。使用简单的加标缓冲溶液和复杂的细胞培养基构建系统并测试阵列。此外，将该系统与生物反应器偶联并测试2周。在一个生物过程中的氧气和pH值检测的预测标准误差分别为0.75%和0.092。在设计的流通布置中的传感器的时间响应对于pH和氧气都小于90秒，完全在细胞培养监测所需的时间内。总的来说，这种感测方法有希望作为芯片实验室类型的传感器，用于完全感测培养环境，并最终可用于反馈系统，该反馈系统允许在没有人为干预的情况下进行监测和控制。(C)2007 Elsevier B.V.保留所有权利。
The sensing of analytes in cell culture media without the introduction of exogenous reagents proves difficult in today's cell culturing systems. In this paper, a completely embeddable microarray sensor that has been developed along with a compact fluorescent imaging system to sense oxygen and pH non-invasively is described. The compact detection system consists of an ultra-bright blue diode source, coupling optics, interference filters and a compact moisture resistant CCD camera. The microarray sensor was created by photoreaction injection molding and contains two separate sensing elements. The system was constructed and the array tested using simple spiked buffer solutions and complex cell media. In addition, the system was coupled to a bioreactor and tested over 2 weeks. The standard error of prediction during a bioprocess for both oxygen and pH detection was 0.75% and 0.092, respectively. The time response of the sensor in the designed flow-through arrangement was less than 90s for both pH and oxygen, well within that needed for cell culture monitoring. Overall, this sensing approach has promise as a lab-on-a-chip type sensor for complete sensing of the culture environment and eventually may be used in a feedback system that allows monitoring and control without human intervention. (C) 2007 Elsevier B.V. All rights reserved.