Salivary biomarkers of periodontal disease in response to treatment.
Salivary biomarkers of periodontal disease in response to treatment.
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DOI：
10.1111/j.1600-051x.2011.01706.x
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发表时间：
2011-05
影响因子：
6.7
通讯作者：
Miller CS
中科院分区：
文献类型：
--
作者：
Sexton WM;Lin Y;Kryscio RJ;Dawson DR 3rd;Ebersole JL;Miller CS
纵向评估牙周炎的唾液生物标志物以确定对治疗的反应。对患有慢性牙周炎的成年人进行了为期6个月的病例对照研究，其中33名参与者单独接受口腔卫生指导（OHI），35名参与者接受洁治和根面平整（SRP）结合OHI。分别于第0、16和28周采集唾液样本，检测白细胞介素1β（IL-1β）、IL-8、巨噬细胞炎性蛋白（MIP）-1α、基质金属蛋白酶-8（MMP-8）、骨保护素（OPG）和肿瘤坏死因子-α（TNF）-α。每次就诊时都会记录牙周病的临床测量结果。到第16周，两组的牙周健康的所有参数均显著改善（p<0.0001），SRP组在第16周和第28周表现出更大的益处。无论治疗组如何，两次随访访视时基线OPG和TNF-α水平均发生显著变化（p<0.03）。仅SRP组的IL-1β和MMP-8水平较基线显著降低（p≤0.04）。与无应答者相比，应答者的OPG、MMP-8和MIP-1α显著降低（分别为p=0.04、0.01、0.05）。在受试者工作特征分析中，MMP-8产生的曲线下面积最高（≥ 0.7; p=0.01）。唾液中IL-1β、MMP-8、OPG和MIP-1α的水平反映了疾病的严重程度和对治疗的反应，表明它们在监测牙周疾病状态方面具有潜在的实用性。
Salivary biomarkers of periodontitis were assessed longitudinally to determine response to therapy. A 6-month case-controlled study of adults with chronic periodontitis was performed, with 33 participants receiving oral hygiene instructions (OHI) alone and 35 with scaling and root planing (SRP) combined with OHI. Saliva samples collected at week 0, 16 and 28 were analyzed for interleukin-1β (IL-1β), IL-8, macrophage inflammatory protein (MIP)-1α, matrix metalloproteinase-8 (MMP-8), osteoprotegerin (OPG) and tumor necrosis factor-α (TNF)-α. Clinical measures of periodontal disease were recorded at each visit. All parameters of periodontal health improved significantly in both groups by week 16 (p<0.0001) with the SRP group demonstrating greater benefit at week 16 and 28. Baseline OPG and TNF-α levels changed significantly at both follow-up visits (p<0.03), regardless of treatment group. IL-1β and MMP-8 levels decreased significantly from baseline (p≤0.04) in the SRP group only. OPG, MMP-8 and MIP-1α were significantly reduced in responders compared with non-responders (p=0.04, 0.01, 0.05 respectively). In receiver operating characteristic analyses, MMP-8 produced the highest area under the curve (≥ 0.7; p=0.01). Salivary levels of IL-1β, MMP-8, OPG and MIP-1α reflected disease severity and response to therapy suggesting their potential utility for monitoring periodontal disease status.