Thin-film optical-acoustic combiner enables high-speed wide-field multi-parametric photoacoustic microscopy in reflection mode.
Thin-film optical-acoustic combiner enables high-speed wide-field multi-parametric photoacoustic microscopy in reflection mode.
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DOI：
10.1364/ol.475373
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发表时间：
2023-01
期刊：
影响因子：
3.6
通讯作者：
Fenghe Zhong;Song Hu
中科院分区：
文献类型：
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作者：
Fenghe Zhong;Song Hu
多参数光声显微镜（PAM）是唯一能够同时高分辨率映射的血氧和血流在体内。然而，其速度受到血流定量所需的密集采样的限制。为了克服这一限制，我们已经开发了一种高速多参数PAM系统，它能够同时采集1000密集采样的B-扫描，通过叠加的快速光学扫描线形状的焦点上的圆柱形聚焦超声换能器在传统的机械扫描的光声双焦点。一种新颖的，据我们所知，光声组合器（OAC）的设计和实施，以适应短的工作距离的换能器，使方便的共焦对准的双焦点反射模式。谐振检流计（GM）提供稳定的高速大角度扫描。这种新系统可以连续监测清醒小鼠大脑中4.5 × 3 mm 2区域内的微血管血氧（sO2）和流量，具有高空间和时间分辨率（分别为6.9 µm和0.3 Hz）。
Multi-parametric photoacoustic microscopy (PAM) is uniquely capable of simultaneous high-resolution mapping of blood oxygenation and flow in vivo. However, its speed has been limited by the dense sampling required for blood flow quantification. To overcome this limitation, we have developed a high-speed multi-parametric PAM system, which enables simultaneous acquisition of ∼500 densely sampled B-scans by superposing the rapid optical scanning across the line-shaped focus of a cylindrically focused ultrasonic transducer over the conventional mechanical scan of the optical-acoustic dual foci. A novel, to the best of our knowledge, optical-acoustic combiner (OAC) is designed and implemented to accommodate the short working distance of the transducer, enabling convenient confocal alignment of the dual foci in reflection mode. A resonant galvanometer (GM) provides stabilized high-speed large-angle scanning. This new system can continuously monitor microvascular blood oxygenation (sO2) and flow over a 4.5 × 3 mm2 area in the awake mouse brain with high spatial and temporal resolutions (6.9 µm and 0.3 Hz, respectively).