The gut bacterial flora associated with brown planthopper is affected by host rice varieties
The gut bacterial flora associated with brown planthopper is affected by host rice varieties
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DOI：
10.1007/s00203-020-02013-8
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发表时间：
2020-09
影响因子：
2.8
通讯作者：
Zheng-Liang Wang;Hai-bo Pan;Wei Wu;Mu-Yu Li;Xiaoping Yu
中科院分区：
文献类型：
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作者：
Zheng-Liang Wang;Hai-bo Pan;Wei Wu;Mu-Yu Li;Xiaoping Yu
肠道菌群在昆虫的发育、进化和环境适应中起着重要作用。褐飞虱（brown planthopper，BPH）是水稻上最具破坏性的害虫之一，但对其肠道微生物区系的研究却知之甚少。在这项研究中，我们调查了肠道细菌群落在两个褐飞虱种群饲养敏感和抗性水稻品种的高通量扩增子测序。我们的结果表明，前列腺增生症的肠道细菌群落具有物种多样性。共捕获29门367属，其中变形菌门和不动杆菌门分别是最突出的门和属。比较分析表明，两种群间肠道细菌群落结构存在显著差异。取食抗病水稻品种的种群物种丰富度显著高于取食感病水稻品种的种群。虽然最优势的肠道细菌在所有分类水平没有表现出显着差异的两个BPH种群之间，两个亚优势门（厚壁菌门和拟杆菌门）和两个亚优势类（拟杆菌和梭菌）的相对丰度显着不同。FAPROTAX分析进一步表明，寄主水稻品种可能引起褐飞虱肠道细菌植物群的变化，两个种群在发酵、甲基营养、木聚糖分解、硝酸盐还原和尿素分解5个代谢相关功能类别上存在显著差异。本研究结果可为研究BPH与其共生微生物之间的相互作用提供参考，也可为未来BPH的生物治理提供依据。
Gut microbiota plays vital roles in the development, evolution and environmental adaptation of the host insects. The brown planthopper (BPH) is one of the most destructive pests of rice, but little is known about its gut microbiota. In this study, we investigated the gut bacterial communities in two BPH populations feeding on susceptible and resistant rice varieties by high-throughput amplicon sequencing. Our results revealed that the gut bacterial communities in BPH were species diverse. A total of 29 phyla and 367 genera were captured, with Proteobacteria andAcinetobacterbeing the most prominent phylum and genus, respectively. Comparative analysis showed that significant differences in the profile of gut bacterial communities existed between the two BPH populations. The species richness detected in the population feeding on the resistant rice variety was significantly higher than that in the population rearing on the susceptible rice variety. Although the most dominant gut bacteria at all taxonomic levels showed no significant differences between the two BPH populations, the relative abundances of two subdominant phyla (Firmicutes and Bacteroidetes) and two subdominant classes (Bacteroidia and Clostridia) were significantly different. FAPROTAX analysis further indicated that host rice varieties might induce changes of the gut bacterial flora in BPH, as significant differences in five metabolism-related functional categories (fermentation, methylotrophy, xylanolysis, nitrate reduction and ureolysis) were detected between the two BPH populations. Our results are informative for studies which focused on the interactions between BPH and its symbiotic microbes and could also provide the basis of future BPH biological management.