Nitric oxide synthase isoforms in the rat uterus: differential regulation during pregnancy and labour.
Nitric oxide synthase isoforms in the rat uterus: differential regulation during pregnancy and labour.
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DOI：
10.1530/jrf.0.1070249
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发表时间：
1996-07
期刊：
影响因子：
--
通讯作者：
Y. Dong;P. Gangula;C. Yallampalli
中科院分区：
文献类型：
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作者：
Y. Dong;P. Gangula;C. Yallampalli
子宫内一氧化氮的产生在怀孕期间增加，在分娩期间减少。在这项研究中，一氧化氮合酶的亚型，这可能是负责一氧化氮生产的变化进行了调查，怀孕和分娩。用单克隆抗体对大鼠子宫内神经元型一氧化氮合酶（NOS I）、巨噬细胞型一氧化氮合酶（NOS II）和内皮细胞型一氧化氮合酶（NOS III）蛋白进行光密度扫描。结果表明：（1）妊娠期大鼠子宫中NOS Ⅱ蛋白浓度显著升高，分娩期大鼠子宫中NOS Ⅱ蛋白浓度显著降低，包括足月和早产（RU 486诱导）;（2）NOS Ⅲ蛋白在所有检测阶段均存在，但浓度无变化;（3）NOS Ⅰ蛋白在非妊娠期大鼠子宫中存在，但在妊娠和分娩期不存在。妊娠和分娩期间子宫NOS II蛋白浓度的变化进一步通过Ca（2+）非依赖性而非Ca（2+）依赖性一氧化氮合酶活性的变化得到证实。因此，增加NOS II，从而在怀孕期间的一氧化氮生产，可能在维持子宫静止的作用。
Production of nitric oxide in the uterus is increased during pregnancy and decreased during delivery. In this study the isoform of nitric oxide synthase which may be responsible for the changes in nitric oxide production was investigated in relation to pregnancy and delivery. Monoclonal antibodies were used to measure changes in neuronal nitric oxide synthase (NOS I), macrophage nitric oxide synthase (NOS II) and endothelial cell nitric oxide synthase (NOS III) protein in the rat uterus by densitometric scanning of specific bands. Results show that: (1) NOS II protein concentrations in the uterus were substantially increased during pregnancy and were decreased during delivery, both at term and preterm (induced by RU486); (2) NOS III protein was present at all stages examined but the concentrations were unchanged; (3) NOS I was present in the rat uterus during the nonpregnant stage but not during pregnancy and delivery. The changes in uterine NOS II protein concentrations during pregnancy and delivery were further confirmed by the changes in the Ca(2+)-independent, but not Ca(2+)-dependent, nitric oxide synthase activity. Therefore, an increase in NOS II, thus in nitric oxide production during pregnancy, may play a role in maintaining uterine quiescence.