Medium-Chain Fatty Acids (MCFA) Production Through Anaerobic Fermentation Using Clostridium kluyveri: Effect of Ethanol and Acetate

Medium-Chain Fatty Acids (MCFA) Production Through Anaerobic Fermentation Using Clostridium kluyveri: Effect of Ethanol and Acetate
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DOI:
10.1007/s12010-017-2674-2
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发表时间:
2018-07-01
影响因子:
3
通讯作者:
Chang, Young-Cheol
Chang, Young-Cheol
中科院分区:
工程技术3区
文献类型:
--
作者:
Reddy, M. Venkateswar;Mohan, S. Venkata;Chang, Young-Cheol

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中链脂肪酸 (MCFA) 是饱和一元羧酸,可用作抗菌剂、腐蚀抑制剂、生物柴油和生物塑料生产的前体。在本研究中,使用克鲁维梭菌 (Clostridium kluyveri) 评估乙酸盐和乙醇的 MCFA 产量。评估了底物、电子供体和甲烷抑制剂对 MCFA 生产的影响。细菌通过链延长过程成功地将乙醇和乙酸盐转化为丁酸盐(C4)、己酸盐(C6)和辛酸盐(C8)。与其他条件相比,在甲烷抑制条件下获得了最高浓度的丁酸盐(4.6 g/l)、己酸盐(3.2 g/l)和辛酸盐(0.5 g/l)。在甲烷抑制条件下,丁酸和己酸的产量分别提高了1.6倍和1.48倍。结果表明,克鲁维梭菌可用于将乙酸盐和乙醇转化为有用的产品,如丁酸盐和己酸盐。
Medium-chain fatty acids (MCFA) are saturated monocarboxylic acids and can be used as antimicrobials, corrosion inhibitors, precursors in biodiesel, and bioplastic production. In the present study, MCFA production was evaluated with acetate and ethanol using the bacteria Clostridium kluyveri. Effects of substrate, electron donor, and methane inhibitor on MCFA production were evaluated. Bacteria successfully converted the ethanol and acetate to butyrate (C4), caproate (C6), and caprylate (C8) by chain elongation process. The highest concentrations of butyrate (4.6 g/l), caproate (3.2 g/l), and caprylate (0.5 g/l) were obtained under methane inhibition conditions than other conditions. The productions of butyrate and caproate were 1.6 and 1.48 times higher under methane inhibition conditions, respectively. Results denoted that the bacteria C. kluyveri can be used for conversion of acetate and ethanol into useful products like butyrate and caproate.