Cell population dynamics (apoptosis, mitosis, and cell-cell communication) during disruption of homeostasis.
Cell population dynamics (apoptosis, mitosis, and cell-cell communication) during disruption of homeostasis.
复制标题
DOI：
10.1006/excr.1999.4771
复制
发表时间：
2000-02
影响因子：
3.7
通讯作者：
Melinda R. Wilson;Troy W. Close;J. Trosko
中科院分区：
文献类型：
--
作者：
Melinda R. Wilson;Troy W. Close;J. Trosko
维持体内平衡的一系列事件必须包括单个细胞内的机制以及群体内细胞之间的相互作用。为了研究这些细胞间和细胞内机制之间的相互作用，在正常二倍体上皮细胞系WB-F344中通过血清剥夺破坏稳态。在群体水平和单个细胞中细胞-细胞通讯（间隙连接功能）的变化使用光漂白测定后的刮擦负载/染料转移和荧光再分布进行监测。细胞凋亡和有丝分裂采用核小体间DNA梯状条带分析和荧光激活细胞分选。结果表明，在早期细胞凋亡和早期有丝分裂的共同要素是持续的间隙连接功能。随着细胞生命（有丝分裂）和细胞死亡（凋亡）的进展，发生了间隙连接功能变化的共同过程。在血清剥夺过程中也观察到伴随细胞凋亡增加的有丝分裂的瞬时刺激。间隙连接可能在这些相反但同样重要的维持体内平衡的机制的启动过程中发挥调节作用。该模型系统为进一步研究细胞内稳态和细胞间稳态之间的关系提供了一个新的模型。
The sequence of events involved in maintenance of homeostasis must encompass mechanisms within single cells as well as interactions between cells within a population. To investigate the interaction among these inter- and intracellular mechanisms, disruption of homeostasis by serum deprivation was performed in WB-F344, a normal diploid epithelial cell line. Changes in cell-cell communication (gap junction function) at the population level and in individual cells were monitored using the scrape load/dye transfer and fluorescence redistribution after photobleaching assays. Apoptosis and mitosis were measured using internucleosomal DNA ladder assays and fluorescence-activated cell sorting. The results indicate that a common element in early apoptosis and early mitosis is sustained gap junction function. As cell life (mitosis) and cell death (apoptosis) progressed, a common process of change in gap junction function occurred. A transient stimulation of mitosis concomitant with increased apoptosis was also observed during serum deprivation. Gap junctions may play a regulatory role during initiation of these opposite yet equally important mechanisms of maintaining homeostasis. This model system is useful for further studies on the relationships among inter- and intracellular mechanisms of homeostasis.