Cell-cell interaction in prostate gene regulation and cytodifferentiation
Cell-cell interaction in prostate gene regulation and cytodifferentiation
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DOI：
10.1073/pnas.94.20.10705
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发表时间：
1997-09-30
影响因子：
11.1
通讯作者：
vandenEngh, G
中科院分区：
文献类型：
--
作者：
Liu, AY;True, LD;vandenEngh, G
为了研究细胞间相互作用对人前列腺细胞分化和基因表达的影响，我们分离了两大上皮细胞群，并将它们与基质细胞联合进行了研究，利用流式细胞术分离了上皮细胞，使用了针对细胞表面差异表达标志物CD44和CD57的抗体，基底上皮细胞表达CD44，管腔上皮细胞表达CD57。CD57（+）管腔细胞是合成前列腺特异性抗原（PSA）的终末分化分泌细胞，PSA的表达受雄激素调控，PSA mRNA是这些细胞中丰富的信息之一，我们发现CD57（+）细胞在前列腺组织被胶原酶分散成单个细胞后，PSA的表达被消除，当CD57（+）细胞与基质细胞重组后，PSA的表达得以恢复。CD44（+）基底细胞具有干细胞的特性，是腔细胞的候选祖细胞，当CD44（+）细胞与基质细胞共培养时，可以检测到通过PSA产生的分化。我们的研究表明，细胞间相互作用在前列腺细胞分化和维持分化状态中起着重要作用。
To examine the role of intercellular interaction on cell differentiation and gene expression in human prostate, we separated the two major epithelial cell populations and studied them in isolation and in combination with stromal cells, The epithelial cells were separated by flow cytometry using antibodies against differentially expressed cell-surface markers CD44 and CD57, Basal epithelial cells express CD44, and luminal epithelial cells express CD57. The CD57(+) luminal cells are the terminally differentiated secretory cells of the gland that synthesize prostate-specific antigen (PSA), Expression of PSA is regulated by androgen, and PSA mRNA is one of the abundant messages in these cells, We show that PSA expression by the CD57(+) cells is abolished after prostate tissue is dispersed by collagenase into single cells, Expression is restored when CD57(+) cells are reconstituted with stromal cells, The CD44(+) basal cells possess characteristics of stem cells and are the candidate progenitors of luminal cells, Differentiation, as reflected by PSA production, can be detected when CD44(+) cells are cocultured with stromal cells. Our studies show that cell-cell interaction plays an important role in prostatic cytodifferentiation and the maintenance of the differentiated state.