Broad-Host Range Gene Transporter Particles Produced by Aliivibrio fischeri
Broad-Host Range Gene Transporter Particles Produced by Aliivibrio fischeri
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DOI：
10.1264/jsme2.me09153
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发表时间：
2009-01-01
影响因子：
2.2
通讯作者：
Kogure, Kazuhiro
中科院分区：
文献类型：
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作者：
Chiura, Hiroshi Xavier;Uchiyama, Nami;Kogure, Kazuhiro
fischeri Aliivibrio NCIMB1281(T) （basonym, Vibrio fischeri）从对数期出芽，自发地开始产生宽宿主范围载体颗粒（AfVP），稳定在7.0 × 10(10)-7.4 × 10（11）粒mL（-1）左右，而宿主种群没有任何伴随变化。afvp呈球形，直径在18.1 ~ 159.2 nm之间变化[中位数+/- SD， 58.4 +/- 11.9 nm, n=528]，其中95.1%的afvp直径在30.2 ~ 84.6 nm之间呈正态分布。浮力密度为1.3607 ~ 1.3980 g cm(-3)， DNA含量为17.3 ~ 95.3 kbp。无论紫外线处理，AfVPs在大肠杆菌AB1157感染约140次的情况下，使电镀效率提高了116-136%。在没有紫外线处理的情况下，观察到每个AfVP有10（-4）到10（-6）个细胞的普遍转导。感染后，颗粒膜留在受体细胞外，出现一种被纤维蛋白样物质包裹的弦状结构。大肠杆菌转导剂（AfV-E-trans）的生长最高可达亲本大肠杆菌受体的约415%。AfV-E-trans获得了产生出芽粒子的能力。
Aliivibrio fischeri NCIMB1281(T) (basonym, Vibrio fischeri) spontaneously started broad-host range vector particle (AfVP) production by budding from the logarithmic phase, and stabilised at around 7.0x10(10)-7.4x10(11) particles mL(-1) without any accompanying change in the host population. AfVPs had a spherical shape and varied in diameter from 18.1 to 159.2 nm [median +/- SD, 58.4 +/- 11.9 nm, n=528], with 95.1% between 30.2 and 84.6 nm in diameter exhibiting a normal distribution. Their buoyant density and DNA content ranged from 1.3607 to 1.3980 g cm(-3), and 17.3 to 95.3 kbp, respectively. Regardless of UV treatment, AfVPs enhanced the efficiency of plating 116-136% at a multiplicity of infection of ca. 140 in Escherichia coli AB1157. Generalised transduction was observed with a frequency of between 10(-4) and 10(-6) cells per AfVP without UV treatment. Upon infection, the particle membrane remained outside the recipient cell, and a string-like structure coated with a fibrous proteinaceous-like material was present. The growth of the E. coli transductant (AfV-E-trans) reached a maximum of ca. 415% that of the parental E. coli recipient. AfV-E-trans acquired the ability to produce budding particles.