Application of "Smart" Amine Donors for Rapid Screening and Scale-Up of Transaminase-Mediated Biotransformations
Application of "Smart" Amine Donors for Rapid Screening and Scale-Up of Transaminase-Mediated Biotransformations
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DOI：
10.1002/ejoc.201800799
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发表时间：
2018-10-17
影响因子：
2.8
通讯作者：
O'Reilly, Elaine
中科院分区：
文献类型：
--
作者：
Gomm, Andrew;Grigoriou, Stylianos;O'Reilly, Elaine
智能胺供体邻苯二甲胺和尸胺用于快速筛选大的酮库，随后使用市售的胺转氨酶ATA 256进行所选化合物的间接规模合成。该方法能够用单一酶进行筛选和重复性规模的生物转化，并简化了富含sp3的小分子文库的生成。
The smart amine donors o-xylylenediamine and cadaverine were employed for the rapid screening of a large ketone library and subsequent preparative-scale synthesis of selected compounds using a commercially available amine transaminase, ATA256. The methodology enables both screening and preparative-scale biotransformations to be performed with a single enzyme and simplifies the generation of sp3-rich small-molecule libraries.