Training-induced elevation in FABP(PM) is associated with increased palmitate use in contracting muscle.
Training-induced elevation in FABP(PM) is associated with increased palmitate use in contracting muscle.
复制标题
训练引起的 FABP(PM) 升高与收缩肌肉时棕榈酸酯的使用增加有关。
DOI：
10.1152/jappl.1999.87.1.285
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Yee,AJ
中科院分区：
文献类型：
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作者：
Turcotte,LP;Swenberger,JR;Tucker,MZ;Yee,AJ
为了评估耐力训练对大鼠脂肪酸代谢的影响，我们测量了16只训练大鼠(T)和18只未训练大鼠（UT）的红肌和白肌的质膜中脂肪酸结合蛋白的含量（FABPPM）和棕榈酸盐的摄取和氧化。在静息和电刺激（ES）期间，给后肢灌注6 mM葡萄糖、1,800 μM棕榈酸盐和[1-14C]棕榈酸盐25分钟。红色肌肉的fabppm含量比白色肌肉高43-226%，训练后红色肌肉的fabppm含量增加了55%。肌肉中琥珀酸脱氢酶活性与fabppm含量呈正相关。在T和UT中，从休息到ES的棕榈酸盐摄取增加了64-73%，T比UT高48-57%，休息时（分别为39.8±3.5∶26.9±4.4 nmol·min - 1·g - 1， T和UT）和ES时（分别为69.0±6.1∶43.9±4.4 nmol·min - 1·g - 1， T和UT）。当大鼠休息时，棕榈酸氧化不受训练的影响；在ES期间，T组棕榈酸酯氧化高于UT组（分别为14.8±1.3 vs 9.3±1.9 nmol·min - 1·g - 1， T和UT组）。综上所述，耐力训练增加了1)静息和收缩灌注肌的血浆游离脂肪酸（FFA）摄取，2)收缩灌注肌的血浆游离脂肪酸氧化，以及3)红肌中fabppm含量。这些结果表明，这些假定的质膜脂肪酸转运蛋白数量的增加可能在训练肌肉中可用，并可能涉及骨骼肌血浆FFA代谢的调节。
To evaluate the effects of endurance training in rats on fatty acid metabolism, we measured the uptake and oxidation of palmitate in isolated rat hindquarters as well as the content of fatty acid-binding proteins in the plasma membranes (FABPPM) of red and white muscles from 16 trained (T) and 18 untrained (UT) rats. Hindquarters were perfused with 6 mM glucose, 1,800 μM palmitate, and [1-14C]palmitate at rest and during electrical stimulation (ES) for 25 min. FABPPMcontent was 43–226% higher in red than in white muscles and was increased by 55% in red muscles after training. A positive correlation was found to exist between succinate dehydrogenase activity and FABPPMcontent in muscle. Palmitate uptake increased by 64–73% from rest to ES in both T and UT and was 48–57% higher in T than UT both at rest (39.8 ± 3.5 vs. 26.9 ± 4.4 nmol ⋅ min−1⋅ g−1, T and UT, respectively) and during ES (69.0 ± 6.1 vs. 43.9 ± 4.4 nmol ⋅ min−1⋅ g−1, T and UT, respectively). While the rats were resting, palmitate oxidation was not affected by training; palmitate oxidation during ES was higher in T than UT rats (14.8 ± 1.3 vs. 9.3 ± 1.9 nmol ⋅ min−1⋅ g−1, T and UT, respectively). In conclusion, endurance training increases1) plasma free fatty acid (FFA) uptake in resting and contracting perfused muscle,2) plasma FFA oxidation in contracting perfused muscle, and3) FABPPMcontent in red muscles. These results suggest that an increased number of these putative plasma membrane fatty acid transporters may be available in the trained muscle and may be implicated in the regulation of plasma FFA metabolism in skeletal muscle.