Superoxide is promoted by sucrose and affects amplitude of circadian rhythms in the evening
Superoxide is promoted by sucrose and affects amplitude of circadian rhythms in the evening
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DOI：
10.1073/pnas.2020646118
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发表时间：
2021-03
期刊：
影响因子：
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通讯作者：
Ángela Román;Xiang Li;Dongjing Deng;J. Davey;Sally James;I. Graham;Michael J. Haydon
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文献类型：
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作者：
Ángela Román;Xiang Li;Dongjing Deng;J. Davey;Sally James;I. Graham;Michael J. Haydon
意义 区分光和糖对光合自养细胞的影响具有挑战性。昼夜节律系统是一个整合光和代谢信号并控制节律性生理和生长的调节网络。我们的实验方法在拟南芥中定义了一个不依赖于光的、糖调节的转录组，并揭示了活性氧（ROS）的一个突出特征。 ROS 是光合作用代谢的副产品，并随昼夜节律振荡，但之前并未被证明可以作为植物昼夜节律振荡器的输入。我们的数据表明，超氧化物作为一种节律性糖信号发挥着作用，它在晚上起作用并影响昼夜节律基因表达和生长。植物必须协调光合代谢与日常环境，并适应节律性生理和发育以匹配碳的有效性。昼夜节律时钟驱动生物节律，从而适应环境线索。光合代谢的产物，包括糖和活性氧（ROS），与植物生物钟密切相关，并且糖已被证明可以向昼夜节律振荡器提供代谢反馈。在这里，我们报告了拟南芥的综合糖调节转录组，并鉴定了与氧化还原和 ROS 过程相关的基因作为转录反应的一个突出特征。我们发现蔗糖会增加超氧化物（O2-）的水平，这是对糖的转录和生长反应所必需的。我们确定了 O2– 调节转录物的昼夜节律，这些转录物在黄昏左右分阶段，并发现 O2– 是蔗糖在晚上促进 TIMING OF CAB1 (TOC1) 表达所必需的。我们的数据揭示了 O2– 作为影响拟南芥昼夜节律振荡器转录控制的代谢信号的作用。
Significance Distinguishing the effects of light and sugars in photoautotrophic cells is challenging. The circadian system is a regulatory network that integrates light and metabolic signals and controls rhythmic physiology and growth. Our experimental approach has defined a light-independent, sugar-regulated transcriptome in Arabidopsis and revealed reactive oxygen species (ROS) as a prominent feature. ROS are byproducts of photosynthetic metabolism and oscillate with circadian rhythms but have not previously been demonstrated as inputs to the plant circadian oscillator. Our data suggest a role for superoxide as a rhythmic sugar signal which acts in the evening and affects circadian gene expression and growth. Plants must coordinate photosynthetic metabolism with the daily environment and adapt rhythmic physiology and development to match carbon availability. Circadian clocks drive biological rhythms which adjust to environmental cues. Products of photosynthetic metabolism, including sugars and reactive oxygen species (ROS), are closely associated with the plant circadian clock, and sugars have been shown to provide metabolic feedback to the circadian oscillator. Here, we report a comprehensive sugar-regulated transcriptome of Arabidopsis and identify genes associated with redox and ROS processes as a prominent feature of the transcriptional response. We show that sucrose increases levels of superoxide (O2–), which is required for transcriptional and growth responses to sugar. We identify circadian rhythms of O2–-regulated transcripts which are phased around dusk and find that O2– is required for sucrose to promote expression of TIMING OF CAB1 (TOC1) in the evening. Our data reveal a role for O2– as a metabolic signal affecting transcriptional control of the circadian oscillator in Arabidopsis.