Abscisic acid induces the expression of AsKIN during the recovery period of garlic cryopreservation
Abscisic acid induces the expression of AsKIN during the recovery period of garlic cryopreservation
复制标题
脱落酸诱导大蒜冻存恢复期AsKIN的表达
DOI：
10.1007/s00299-022-02894-7
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发表时间：
2022-09-06
影响因子：
6.2
通讯作者：
Wu，Zhen
中科院分区：
文献类型：
--
作者：
Xing，Xiaodong;Liu，Min;Wu，Zhen
关键信息在大蒜低温保存恢复期间，脱落酸诱导AsKIN的表达。AsKIN被鉴定为参与抗冷和渗透胁迫的基因。低温保存已被证明可以有效去除大蒜中的病毒。然而，冷冻保存中的氧化损伤对保存后的存活率有显着影响。脱落酸 (ABA) 已被证明可以减少氧化应激并促进冷冻保存后的存活。然而，尚不清楚哪些基因在此过程中发挥重要作用。在本研究中，我们在脱水步骤中添加了 ABA，并分析了 ABA 处理组和对照组在三个低温步骤（脱水、卸载和恢复）中的转录组差异。通过短时间序列表达挖掘器（STEM）分析和加权基因共表达网络分析（WGCNA），将恢复步骤确定为低温保存中基因表达水平发生显着变化的时期。 ABA的添加促进了恢复步骤中微管相关基因的表达上调。我们进一步鉴定出AsKINA是恢复步骤中的枢纽基因并验证了其功能。结果表明，AsKIN的过表达增强了拟南芥对低温氧化胁迫的耐受性，影响了响应冷胁迫和渗透胁迫的基因表达，促进了胁迫后植物的生长。 AsKIN 基因可能参与植物对冷胁迫和渗透胁迫的反应。这些结果揭示了ABA在冷冻保存中的分子机制，并阐明了kinesin-14亚家族的潜在生物学功能。
Key messageAbscisic acid induced the expression ofAsKINduring the recovery period of garlic cryopreservation.AsKINwas identified as a gene involved in cold and osmotic stress resistance.Cryopreservation has been proven to be effective in removing viruses from garlic. However, oxidative damage in cryopreservation has a significant impact on the survival after preservation. Abscisic acid (ABA) has been shown to reduce oxidative stress and promote the survival after cryopreservation. However, it is not clear which genes play important roles in this process. In this study, we added ABA to the dehydration step and analyzed the transcriptomic divergences between the ABA-treated group and the control group in three cryogenic steps (dehydration, unloading and recovery). By short time-series expression miner (STEM) analysis and weighted gene co-expression network analysis (WGCNA), the recovery step was identified as the period of significant changes in gene expression levels in cryopreservation. The addition of ABA promoted the upregulated expression of microtubule-related genes in the recovery step. We further identifiedAsKINas a hub gene in the recovery step and verified its function. The results showed that overexpression ofAsKINenhanced the tolerance ofArabidopsisto oxidative stress in cryopreservation, influenced the expression of genes in response to cold and osmotic stress and promoted plant growth after stress. TheAsKINgene is likely to be involved in the plant response to cold stress and osmotic stress. These results reveal the molecular mechanisms of ABA in cryopreservation and elucidate the potential biological functions of the kinesin-14 subfamily.