Using restriction mapping to teach basic skills in the molecular biology lab
Using restriction mapping to teach basic skills in the molecular biology lab
复制标题
使用限制性图谱教授分子生物学实验室的基本技能
DOI：
10.1002/bmb.45
复制
发表时间：
2007
影响因子：
1.4
通讯作者：
E. D. De Stasio
中科院分区：
文献类型：
--
作者：
L. Walsh;Elizabeth Shaker;E. D. De Stasio
用限制性酶消化 DNA、计算反应试剂的体积和浓度以及通过琼脂糖凝胶电泳分离 DNA 片段是常见的分子生物学技术，最好通过重复进行教授。以下关于质粒限制性图谱的开放式研究性实验室练习使学生能够获得技术专业知识，同时探索凝胶电泳和限制性图谱的实用性。由于其解释性质，该项目还提供适合书面报告的数据，因此可用于强化图形演示和科学写作技能的课程。
Digestion of DNA with restriction enzymes, calculation of volumes and concentrations of reagents for reactions, and the separation of DNA fragments by agarose gel electrophoresis are common molecular biology techniques that are best taught through repetition. The following open‐ended, investigative laboratory exercise in plasmid restriction mapping allows students to gain technical expertise while simultaneously exploring the utility of gel electrophoresis and restriction mapping. Because of its interpretive nature, this project also provides data suitable for a written report, and can thus be used to reinforce lessons on figure presentation and science writing skills.