The role of neutrophils and oxygen free radicals in post-operative adhesions
The role of neutrophils and oxygen free radicals in post-operative adhesions
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DOI：
10.1016/j.jss.2006.05.006
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发表时间：
2006-11-01
影响因子：
2.2
通讯作者：
Jeekel, Hans
中科院分区：
文献类型：
--
作者：
ten Raa, Sander;van den Tol, M. Petrousjka;Jeekel, Hans
背景术后腹腔内粘连的形成仍然是一个主要的外科问题。手术引起炎症。反应，这是负责粘附形成。中性粒细胞及其氧自由基是术后早期炎症反应的关键介质。本研究评价了通过清除氧自由基阻断中性粒细胞或其产物流入对粘附形成的影响。材料和方法。使用可复制的大鼠模型诱导术后腹腔内粘连。在第一个实验中，抗中性粒细胞血清（ANS）用于防止手术后中性粒细胞进入腹膜腔。在第二个实验中，超氧化物歧化酶（SOD），过氧化氢酶和甘露醇进行了测试，以消除超氧化物，过氧化氢和羟基自由基，分别。在阳性对照组中，69 - 76%的关注区域含有粘连。在所有实验组中，除甘露醇外，均可显着减少术后粘连形成。ANS使粘附形成减少38%（P < 0.001），SOD/CAT减少42%（P < 0.01）。手法不能减少粘连的形成。腹膜创伤后腹腔内中性粒细胞的流入在术后粘连形成中起重要作用。在术后早期炎症反应中防止腹腔内中性粒细胞的流入可以减少粘连形成，但是通过清除其产物的更具选择性的方法被证明是有效的。(c)2006年爱思唯尔公司All rights reserved.
Background. Postoperative intra-abdominal adhesion formation remains a major surgical problem. Surgery induces an inflammatory. reaction, which is responsible for adhesion formation. Neutrophils and their oxygen-free radicals are key mediators in the early post-operative inflammatory response. The present study evaluates the effect of either blocking the influx of neutrophils or its products by scavenging oxygen-free radicals on adhesion formation.Materials and methods. Reproducible rat models were used to induce post-surgical intra-abdominal adhesions. In the first experiment anti-neutrophil serum (ANS) was used to prevent neutrophils from entering the peritoneal cavity after surgery. In a second experiment superoxide dismutase (SOD), catalase, and mannitol were tested, to scavenge the superoxide, hydrogen peroxide, and hydroxyl radicals, respectively.Results. In positive control groups 69 to 76% of the area of interest contained adhesions. In all experimental groups, except for mannitol, a significant reduction in post-surgical adhesion formation could be achieved. ANS reduced adhesion formation by 38% (P < 0.001) and SOD/catalase by 42% (P < 0.01). Mannitol could not reduce adhesion formation.Conclusions. Intra-abdominal influx of neutrophils after surgical peritoneal trauma plays an important role in post-operative adhesion formation. Preventing the intra-abdominal influx of neutrophils in the early post-operative inflammatory reaction can reduce adhesion formation, but an even more selective approach, by scavenging its products, proved as efficient. (c) 2006 Elsevier Inc. All rights reserved.