MUTATIONS THAT ALTER THE DNA-SEQUENCE SPECIFICITY OF THE CATABOLITE GENE ACTIVATOR PROTEIN OF ESCHERICHIA-COLI
MUTATIONS THAT ALTER THE DNA-SEQUENCE SPECIFICITY OF THE CATABOLITE GENE ACTIVATOR PROTEIN OF ESCHERICHIA-COLI
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DOI：
10.1038/311232a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
BECKWITH, J
中科院分区：
文献类型：
--
作者：
EBRIGHT, RH;COSSART, P;BECKWITH, J
分离出三种突变，这些突变改变了分解代谢基因激活蛋白（CAP）的 DNA 序列特异性，从 AA-TGTGA--T---TCA-ATW 变为 AA-TGTAA--T---TCA-ATW。所有 3 个突变都影响 CAP 的相同氨基酸，即谷氨酸 181。这显然是 CAP 中与对称识别位点的碱基对 7 和 16 接触的氨基酸。
Three mutations that alter the DNA sequence specificity of the catabolite gene activator protein (CAP) from AA-TGTGA--T---TCA-ATW to AA-TGTAA--T---TCA-ATW were isolated. All 3 mutations affect the same amino acid of CAP, glutamic acid 181. This apparently is the amino acid of CAP that makes contacts with base pairs 7 and 16 of the symmetrical recognition site.