Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
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DOI：
10.1038/nbt1318
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发表时间：
2007-07-01
影响因子：
46.9
通讯作者：
Zhang, Weidong
中科院分区：
文献类型：
--
作者：
Adewumi, Oluseun;Aflatoonian, Behrouz;Zhang, Weidong
国际干细胞倡议对来自全世界17个实验室的59个人类胚胎干细胞系进行了鉴定。尽管不同的基因型和不同的技术用于衍生和维护，所有线表现出类似的表达模式的几个标记的人胚胎干细胞。它们表达糖脂抗原SSEA 3和SSEA 4，硫酸角质素抗原TRA-1-60、TRA-1-81、GCTM 2和GCT 343，以及蛋白质抗原CD 9、Thy 1（也称为CD 90）、组织非特异性碱性磷酸酶和1类HLA，以及强发育调节基因NANOG、POU 5 F1（以前称为0 CT 4）、TDGF 1、DNMT 3B、GABRB 3和GDF 3。然而，这些品系并不相同：几个谱系标记物的表达差异是明显的，几个印迹基因表现出大致相似的等位基因特异性表达模式，但观察到一些基因依赖性变异。此外，一些雌性品系表达容易检测到的XIST水平，而另一些则没有。未检测到支原体、细菌或致细胞病变病毒对管路的显著污染。
The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.