Effect of dietary administration of probiotics on growth and intestine functionality of juvenile Senegalese sole (Solea senegalensis, Kaup 1858)
Effect of dietary administration of probiotics on growth and intestine functionality of juvenile Senegalese sole (Solea senegalensis, Kaup 1858)
复制标题
DOI：
10.1111/j.1365-2095.2008.00581.x
复制
发表时间：
2009-04-01
影响因子：
3.5
通讯作者：
Moyano, F. J.
中科院分区：
文献类型：
--
作者：
De Rodriganez, M. A. Saenz;Diaz-Rosales, P.;Moyano, F. J.
通过测量塞内加尔鳎幼鱼的生长和饲料效率、亮氨酸氨肽酶和碱性磷酸酶的活性以及肠道结构变化，评估了来自交替单胞菌科的两种细菌益生菌菌株（Ppd11和Pdp13）连续60天的饮食施用效果。此外，通过变性梯度凝胶电泳研究了肠道微生物群的概况。接受益生菌的鱼的生长和营养利用率显着高于喂食对照饮食的鱼。尽管在接受 Pdp13 的鱼中测量到更高的脂质肌肉含量，但在处理之间的近端成分没有观察到差异。与 Ppd11 和对照组相比，这些鱼还表现出更高的 AP 活性。肠道微生物群的特征清楚地将接受益生菌的鱼与对照组的鱼区分开来。显微镜检查显示，接受对照饮食的鱼的肠细胞中存在脂滴积聚，但饲喂益生菌的鱼则没有。讨论了这些结构变化和增长绩效之间的相互作用。
The effects of the dietary administration of two bacterial probiotic strains (Ppd11 and Pdp13) from the Alteromonadaceae family for 60 days, were assessed by measuring growth and feed efficiency, activities of leucine aminopeptidase and alkaline phosphatase and structural changes in the intestine of juvenile Senegalese sole. In addition, the profile of intestinal microbiota was studied by Denaturing Gradient Gel Electrophoresis. Growth and nutrient utilization were significantly higher in fish receiving probiotics than in those fed the control diet. No differences were observed in proximal composition between treatments, though higher lipid muscle content was measured in fish receiving Pdp13. Those fish also exhibited higher activities of AP when compared to Ppd11 and control groups. The profile of intestinal microbiota clearly separated those fish receiving probiotics from those of the control group. Microscopical examination revealed accumulation of lipid droplets in the enterocytes of fish receiving the control diet, but not in those fed on probiotics. Interactions between those structural changes and growth performance are discussed.