ORFs and genes: how strong a connection?
ORFs and genes: how strong a connection?
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DOI：
10.1089/cmb.1995.2.117
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
Fickett, J W
中科院分区：
文献类型：
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作者：
Fickett, J W
开放阅读框（ORF）的长度是定位新基因的一个重要证据，特别是在剪接罕见的生物体中。然而，目前还没有系统的研究量化ORF长度与基因功能可能性之间的相关程度。在本文中，基于数据库和模拟的证据，导出了估计给定ORF长度的基因功能条件概率的技术。目前已经对酿酒酵母的几个完整染色体进行了测序，并且正在花费大量的努力来定位和表征这些序列中的基因。因此，我们说明了这种有机体的技术。
The length of an open reading frame (ORF) is one important piece of evidence often used in locating new genes, particularly in organisms where splicing is rare. However, there have been no systematic studies quantifying the degree of correlation between length of ORF, on the one hand, and likelihood of gene function, on the other. In this paper, techniques are derived to estimate the conditional probability of gene function, given ORF length, based on evidence both from the databases and from simulation. Several complete chromosomes of Saccharomyces cerevisiae have now been sequenced, and considerable effort is being expended on locating and characterizing the genes in these sequences. Thus, we illustrate the techniques for this organism.