Dimethyl sulfoxide as an inducer of differentiation in preosteoblast MC3T3-E1 cells
Dimethyl sulfoxide as an inducer of differentiation in preosteoblast MC3T3-E1 cells
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DOI：
10.1016/j.febslet.2005.11.062
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发表时间：
2006-01-09
期刊：
影响因子：
3.5
通讯作者：
Kung, AWC
中科院分区：
文献类型：
--
作者：
Cheung, WMW;Ng, WW;Kung, AWC
成骨细胞分化是骨形成的重要组成部分。二甲基亚砜(DMSO)是一种可溶于水的溶剂，广泛用于成骨细胞研究中的受体配体。然而，对其对成骨前体细胞的影响却知之甚少。在本研究中，我们利用小鼠成骨前细胞系MC3T3-E1细胞，证明了DMSO通过激活Runx2和Osterix有效地诱导MC3T3-E1细胞向成骨细胞分化，并依赖于蛋白激酶C(PKC)途径。我们进一步证明，PKC通路的长时间激活足以诱导成骨细胞分化，可能是通过激活PKD/PKCp。(C)2005年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Osteoblastic differentiation is an essential part of bone formation. Dimethyl sulfoxide (DMSO) is a water miscible solvent that is used extensively for receptor ligands in osteoblast studies. However, little is known about its effects on osteoblastogenic precursor cells. In this study, we have used a murine preosteoblast cell line MC3T3-E1 cells to demonstrate that DMSO effectively induces osteoblastic differentiation of MC3T3-E1 cells via the activation of Runx2 and osterix and is dependent upon the protein kinase C (PKC) pathways. We further demonstrated that prolonged activation of PKC pathways is sufficient to induce osteoblastic differentiation, possibly via the activation of PKD/PKCp. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.