Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis
Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis
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DOI：
10.1007/s00425-005-0099-6
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发表时间：
2006-02-01
期刊：
影响因子：
4.3
通讯作者：
Hwang, BK
中科院分区：
文献类型：
--
作者：
Hong, JK;Hwang, BK
使用农杆菌介导的瞬时表达测定来检查作为细菌感染和渗透胁迫的结果的CAChi 2启动子的激活。在CAChi 2基因的上游基因组序列中发现了几个与胁迫相关的顺式作用元件。在用CAChi 2启动子-β-葡萄糖醛酸苷酶（GUS）基因瞬时转化的烟草叶片组织中，CAChi 2启动子被假单胞菌烟草致病变种（Pseudomonaserumingae pv.）烟粉虱感染CAChi 2-GUS的激活与渗透胁迫密切相关，包括甘露醇和NaCl处理。-378 CAChi 2启动子足以通过水杨酸处理诱导CAChi 2基因。转基因拟南芥中CAChi 2的过表达增强了对细菌性病害的抗性。番茄感染CAChi 2过表达拟南芥植物也表现出增加耐NaCl诱导的渗透胁迫在种子萌发和幼苗生长。在NaCl胁迫不存在的情况下，CAChi 2过表达诱导NaCl胁迫响应基因RD 29 A的表达。在种子萌发过程中，CAChi 2过表达的转基因植物表现出对脱落酸的敏感性增加。
The activation of the CAChi2 promoter as the result of bacterial infection and osmotic stresses was examined using the Agrobacterium-mediated transient expression assay. Several stress-related cis-acting elements were revealed within the upstream genomic sequence of the CAChi2 gene. In tobacco leaf tissues transiently transformed with the CAChi2 promoter-beta-glucuronidase (GUS) gene, the CAChi2 promoter was up-regulated by Pseudomonas syringae pv. tabaci infection. The CAChi2-GUS activation was closely related to osmotic stresses, including treatment with mannitol and NaCl. The -378 CAChi2 promoter was sufficient for the CAChi2 gene induction by salicylic acid treatment. CAChi2 overexpression in the transgenic Arabidopsis plants enhanced bacterial disease resistance against Pseudomonas syringae pv. tomato infection. CAChi2-overexpressing Arabidopsis plants also exhibited increased tolerance to NaCl-induced osmotic stresses during seed germination and seedling growth. CAChi2 overexpression induced the expression of the NaCl stress-responsive gene RD29A in the absence of NaCl stress. The CAChi2-overexpressing transgenic plants exhibited increased sensitivity to abscisic acid during seed germination.