Effect of atriopeptin II on isolated opercular epithelium of Fundulus heteroclitus.
Effect of atriopeptin II on isolated opercular epithelium of Fundulus heteroclitus.
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atriopeptin II 对异斜眼底离体岛盖上皮的影响。
DOI：
10.1152/ajpregu.1988.254.1.r27
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Zadunaisky,JA
中科院分区：
文献类型：
--
作者：
Scheide,JI;Zadunaisky,JA
采用短路电流技术观察了心房肽Ⅱ（ANF）对体外培养的大鼠鳃盖上皮细胞的作用。血清中添加ANF刺激氯化物分泌（短路电流）19%以上的控制值与7%的组织电导增加。将ANF粘液添加到鳃盖上皮没有效果。ANF刺激的电流具有剂量依赖性，在10（-7）M时达到最大值。ANF的加入没有影响电流或电导的鳃盖上皮浸泡在无氯林格氏。异丙肾上腺素（10（-6）M）可使鳃盖电流高于心钠素反应。用普萘洛尔（10（-5）M）预处理鳃盖上皮不能抑制ANF对短路电流的刺激，但能抑制异丙肾上腺素反应，表明ANF的刺激活性不依赖于β-肾上腺素能受体。在用河豚毒素（10（-6）或10（-5）M）或地尔硫卓（10（-4）M）预处理的鳃盖中发现ANF刺激的短路电流，表明ANF反应不是由于神经刺激。用二丁酰腺苷3 '，5'-环一磷酸、8-溴腺苷3 '，5'-环一磷酸或8-溴鸟苷3 '，5'-环一磷酸（10（-4）M）预处理鳃盖组织对ANF刺激反应没有影响。短期淡水适应的鳉鱼的鳃盖上皮也显示出ANF诱导的反应。ANF对异基底氯细胞氯分泌的刺激可能在硬骨鱼类离子调节中起作用。
The effect of atriopeptin II (ANF) on the in vitro opercular epithelium was investigated by use of short-circuit current techniques. Serosal addition of ANF stimulates chloride secretion (short-circuit current) 19% above control values with a 7% increase in tissue conductance. Mucosal addition of ANF to the opercular epithelium was without effect. The ANF stimulation of the current was dose dependent with a maximum at 10(-7) M. The addition of ANF had no effect on the current or the conductance of opercular epithelia bathed in Cl--free Ringer. The opercular current could be stimulated above the ANF response by isoproterenol (10(-6) M). Pretreatment of the opercular epithelium with propranolol (10(-5) M) did not inhibit the stimulation of the short-circuit current by ANF but did inhibit the isoproterenol response indicating that the ANF stimulatory activity was independent of the beta-adrenergic receptors. The ANF-stimulated short-circuit current was found in operculi pretreated with tetrodotoxin (10(-6) or 10(-5) M) or diltiazem (10(-4) M) indicating the ANF response was not due to nerve stimulation. Pretreatment of opercular tissue with dibutyryl adenosine 3',5'-cyclic monophosphate, 8-bromoadenosine 3',5'-cyclic monophosphate, or 8-bromoguanosine 3',5'-cyclic monophosphate (10(-4) M) had no effect on the ANF stimulatory response. Opercular epithelia from short-term freshwater-adapted killifish also showed the ANF-induced response. The stimulation of chloride secretion in Fundulus heteroclitus chloride cells by ANF may have a role in teleost ion regulation.