TRANSIENT EXPRESSION OF THE ANGIOTENSIN-II RECEPTOR - A RAPID AND FUNCTIONAL-ANALYSIS OF A CALCIUM-MOBILIZING 7-TRANSMEMBRANE DOMAIN RECEPTOR IN COS-7 CELLS
TRANSIENT EXPRESSION OF THE ANGIOTENSIN-II RECEPTOR - A RAPID AND FUNCTIONAL-ANALYSIS OF A CALCIUM-MOBILIZING 7-TRANSMEMBRANE DOMAIN RECEPTOR IN COS-7 CELLS
复制标题
DOI：
10.1016/0006-291x(89)92693-4
复制
发表时间：
1989-12-29
影响因子：
3.1
通讯作者：
PAYAN, DG
中科院分区：
文献类型：
--
作者：
MCGILLIS, JP;SUDDUTHKLINGER, J;PAYAN, DG
将mas癌基因/血管紧张素II受体亚克隆到哺乳动物表达载体pCDM 8中，并用于瞬时转染猴肾衍生的COS-7细胞。结果，mas转染的COS-7细胞表达功能性血管紧张素II受体，其能够转导血管紧张素II刺激后细胞内Ca 2+的增加。血管紧张素II刺激的Ca 2+变化可以在转染后24小时在荧光计和荧光激活细胞分选仪中测量。这些结果描述了一种快速的方法，7-跨膜结构域受体基因的功能分析。
The mas oncogene/angiotensin II receptor was subcloned into a mammalian expression vector pCDM8 and used to transiently transfect monkey kidney derived COS-7 cells. As a result, the mas transfected COS-7 cells expressed a functional angiotensin II receptor capable of transducing an increase in intracellular Ca2+ following stimulation with angiotensin II. The angiotensin II stimulated changes in Ca2+ could be measured 24 hours after transfection in both a fluorimeter and a fluorescence activated cell sorter. These results describe a rapid method for the functional analysis of the 7-transmembrane domain receptor genes.