Sharing of an HLA-B27-restricted H-Y antigen between rat and mouse.
Sharing of an HLA-B27-restricted H-Y antigen between rat and mouse.
复制标题
大鼠和小鼠之间共享 HLA-B27 限制性 H-Y 抗原。
DOI：
10.1007/bf00210477
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发表时间：
1993
期刊：
影响因子：
3.2
通讯作者：
Breban,M
中科院分区：
文献类型：
--
作者：
Simmons,WA;Taurog,JD;Hammer,RE;Breban,M
本工作的目的有两个：1.研究转HLA-B27和人β2-微球蛋白基因HB 2 M的大鼠是否能产生B27限制性细胞毒性T淋巴细胞（CTL）对雄性H-Y抗原的反应; 2.研究这种CTL是否能识别HLA-B27背景下的大鼠和小鼠H-Y。B27/HB 2 M转基因系21-4L的雌性大鼠用来自相同系的雄性的细胞在体内致敏。用21-4L雄性大鼠或B27/HB 2 M转基因56-3系雄性小鼠的辐射抗原提呈细胞培养这些雌性小鼠的淋巴结细胞，产生CTL效应物。CTL表现出雄性特异性、B27特异性的大鼠和小鼠靶标裂解。通过对B27或大鼠CD 8具有特异性的单克隆抗体，可使B27靶点裂解。雄性B27大鼠和小鼠靶标的特异性裂解同样可通过大鼠或小鼠雄性B27冷靶标进行复制，但不显著通过雌性或非转基因冷靶标。B27限制性CTL既不识别也不被B27+或B27-男性或女性人类靶标抑制。这些结果表明，CD 8+、B27限制性抗H-Y CTL识别大鼠和小鼠中的H-Y肽抗原并在进化上保守。此外，他们建立了转基因大鼠作为模型系统，用于检查T细胞对I类HLA分子呈递的抗原的应答。
The purpose of this work was twofold: 1 to learn whether rats transgenic forHLA-B27and the human β2-microglobulin geneHB2Mcan mount B27-restricted cytolytic T lymphocyte (CTL) responses to the male H-Y antigen, and 2 to learn whether such CTLs would recognize both rat and mouse H-Y in the context of HLA-B27. Female rats of theB27/HB2Mtransgenic line 21-4L were primed in vivo with cells from males of the same line. CTL effectors were generated from lymph node cells of these females following culture with irradiated antigen-presenting cells from either male 21-4L rats or male mice of theB27/HB2Mtransgenic 56-3 line. The CTLs showed male-specific, B27-specific lysis of both rat and mouse targets. Lysis of B27 targets was inhibitable by monoclonal antibodies specific for B27 or rat CD8. Specific lysis of male B27 rat and mouse targets was inhibitable equally by either rat or mouse male B27 cold targets, but not significantly by female or nontransgenic cold targets. The B27-restricted CTLs neither recognized nor were inhibited by B27+or B27-male or female human targets. These results demonstrate that CD8+, B27-restricted, anti-H-Y CTLs recognize and evolutionarily conserved H-Y peptide antigen in both rats and mice. In addition, they establish the transgenic rat as a model system for examining the T-cell response to antigen presented by class I HLA molecules.