Eukaryotic communities associated with the decomposition of rice straw compost in a Japanese rice paddy field estimated by DGGE analysis
Eukaryotic communities associated with the decomposition of rice straw compost in a Japanese rice paddy field estimated by DGGE analysis
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DOI：
10.1007/s00374-007-0239-1
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发表时间：
2008-02-01
影响因子：
6.5
通讯作者：
Asakawa, Susumu
中科院分区：
文献类型：
--
作者：
Hatamoto, Masashi;Tanahashi, Takanori;Asakawa, Susumu
为了估计水稻种植期间淹水条件下负责稻草堆肥分解的真核生物群落的演替和系统发育组成，在日本稻田中进行了针对 18S rDNA 的变性梯度凝胶电泳 (DGGE) 分析和测序。淹水稻田稻草堆肥中的真核生物群落受中季排水影响，主要由真菌类（子囊菌门、接合菌门、壶菌门）和原生动物（纤毛门、裸足门、指足门）组成，大部分在水稻种植期间持续存在。结果表明，这些真核成员与稻田土壤中稻草堆肥的分解直接或间接相关。
To estimate the succession and phylogenetic composition of the eukaryotic communities responsible for the decomposition of rice straw compost under flooded conditions during the cultivation period of paddy rice, denaturing gradient gel electrophoresis (DGGE) analysis targeting 18S rDNA followed by sequencing was conducted in a Japanese paddy field. The eukaryotic communities in rice straw compost incorporated into the flooded paddy field were influenced by the mid-season drainage and mainly composed of fungi (Ascomycota, Zygomycota, and Chytridiomycota) and protozoa (Ciliophora, Euglyphida, and Dactylopodida), most of which existed continuously during the cultivation period of paddy rice. The results indicated that these eukaryotic members were associated with the decomposition of rice straw compost in paddy field soil directly or indirectly.