Use of an Infectious cDNA Clone of Pepper Veinal Mottle Virus to Confirm the Etiology of a Disease in Capsicum chinense
Use of an Infectious cDNA Clone of Pepper Veinal Mottle Virus to Confirm the Etiology of a Disease in Capsicum chinense
复制标题
利用辣椒脉斑驳病毒感染性 cDNA 克隆确认辣椒疾病的病因
DOI：
10.1094/phyto-08-19-0307-fi
复制
发表时间：
2020-01-01
期刊：
影响因子：
3.2
通讯作者：
Liu, Wenbo
中科院分区：
文献类型：
--
作者：
Hu, Weiyao;Qin, Li;Liu, Wenbo
黄灯笼辣椒品种是最辣的辣椒品种之一，是中国海南岛特有的黄灯笼辣椒（Capsicum chinense）地方种质资源。然而，该品种易感染严重影响其产量的病毒病害。在本研究中，我们报道辣椒脉斑驳病毒（PVMV）与黄灯笼辣椒的叶片褪绿和皱缩症状有关。为了验证这种相关性，我们构建了一个名为HNu的PVMV分离物的全长cDNA克隆。源自该cDNA克隆的病毒子代在辣椒中进行系统性复制和移动，诱导出与田间黄灯笼辣椒中PVMV - HNu所诱导的相同症状。结果表明PVMV - HNu是黄灯笼辣椒叶片褪绿和皱缩病的病原。这一认识将有助于PVMV所致病害的诊断和预防。此外，该cDNA克隆可作为一种反向遗传学工具，用于研究PVMV的分子致病机制。
The pepper cultivar Yellow Lantern, one of the spiciest pepper varieties, is a local germplasm of Capsicum chinense, cultivated exclusively on Hainan Island, China. However, this variety is susceptible to viral diseases that severely affect its production. In this study, we report that pepper veinal mottle virus (PVMV) is associated with foliar chlorosis and rugosity symptoms in Yellow Lantern. To verify this correlation, we constructed a full-length cDNA clone of a PVMV isolate named HNu. The virus progeny derived from the cDNA clone replicated and moved systemically in the pepper, inducing the same symptoms as those induced by PVMV-HNu in Yellow Lantern peppers in the field. The results support that PVMV-HNu is the causal agent of foliar chlorosis and rugosity disease in Yellow Lantern. This knowledge will help in the diagnosis and prevention of disease caused by PVMV. Furthermore, the cDNA clone serves as a reverse genetic tool to study the molecular pathogenesis of PVMV.