DACS, novel matrix structure composed of chondroitin sulfate proteoglycan in the brain
DACS, novel matrix structure composed of chondroitin sulfate proteoglycan in the brain
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DOI：
10.1016/j.bbrc.2007.10.040
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发表时间：
2007-12-14
影响因子：
3.1
通讯作者：
Wanaka, Akio
中科院分区：
文献类型：
--
作者：
Hayashi, Noriko;Tatsumi, Kouko;Wanaka, Akio
硫酸软骨素蛋白聚糖（CSPG）是大脑细胞外基质（ECM）的主要成分。在成人大脑皮层中，有一种特殊的含有 CSPG 的结构，称为神经周围网 (PNN)，它是高度浓缩的 ECM 结构。在这里，我们报告了一种与成年小鼠大脑皮层中的 PNN 不同的新的包含 CSPG 的结构。抗硫酸软骨素抗体 CS56 描绘出具有独特形态的结构，如蒲公英时钟。因此，我们将其命名为 DAndelion Clock-like Structure (DACS)。免疫组织化学证据表明，DACS 包围着一组 NeuN 阳性/GABA 阴性神经元。在超微结构水平上，CS56 免疫反应性位于星形胶质细胞的细胞质和膜上。随着出生后大脑皮层的成熟，DACS 在关键期结束时变得可见。这是第一份报告，证明成人大脑皮层中一组皮质神经元周围存在由 CSPG 组成的 ECM 结构 DACS。 (C) 2007 Elsevier Inc. 保留所有权利。
Chondroitin sulfate proteoglycans (CSPGs) are major components of the extracellular matrix (ECM) in the brain. In the adult cerebral cortex, there are special CSPG-containing structures known as perineuronal nets (PNNs), which are highly condensed ECM structures. Here, we report a novel CSPG-containing structure distinct from PNNs in the adult mouse cerebral cortex. An anti-chondroitin sulfate antibody CS56 delineated a structure with a unique morphology like a dandelion clock. Accordingly, we named it DAndelion Clock-like Structure (DACS). Immunohistochemical evidence showed that DACSs surrounded a group of NeuN-positive/GABA-negative neurons. At ultrastructural level, CS56-immunoreactivities were localized in the cytoplasm and on the membrane of astrocytes. As the postnatal cerebral cortex matured, DACSs became visible around the end of the critical period. This is the first report demonstrating the presence of an ECM structure DACS composed of CSPGs around a group of cortical neurons in the adult cerebral cortex. (C) 2007 Elsevier Inc. All rights reserved.