Effects of apatite, transforming growth factor β-1, bone morphogenetic protein-2 and interleukin-7 on ameloblast differentiation in vitro
Effects of apatite, transforming growth factor β-1, bone morphogenetic protein-2 and interleukin-7 on ameloblast differentiation in vitro
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DOI：
10.1046/j.0909-8836.1999.eos107611.x
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发表时间：
1999-12-01
影响因子：
1.9
通讯作者：
Ruch, JV
中科院分区：
文献类型：
--
作者：
Coin, R;Haïkel, Y;Ruch, JV
前成釉细胞与无细胞前牙本质-牙本质复合后在体外可明显分化。能够触发成釉细胞终末分化的前牙本质-牙本质组分尚未被鉴定，但一些生长因子（TGF β超家族成员）已被证明与该基质相关，并且原位杂交已证明在面对分化的成釉细胞的成牙本质细胞中存在TGF β和BMP-2的转录物。此外，已报道极化成釉细胞中细胞因子白细胞介素-7（IL-7）的受体的强烈表达。在这项研究中，分离的E-18和E-19小鼠磨牙釉质器官在BMP-2，TGF β-1，IL-7或合成磷灰石的存在下体外培养。TGF-β 1和BMP-2联合肝素诱导成釉细胞分化。IL-7维持成釉细胞的极化状态。BMP-2浸泡的磷灰石诱导成釉细胞的功能分化（分泌釉原蛋白）。结合这些数据与以前的工作，一个工作假设有关的成釉细胞终末分化的控制：成牙本质细胞分泌的TGF β超家族的成员可能被困的前牙本质成分，然后由牙本质磷灰石，这些生长因子可能会触发成釉细胞终末分化的细胞功能序列。
Preameloblasts overtly differentiate in vitro when recombined with cell-free predentin-dentin. The predentin-dentin components able to trigger ameloblast terminal differentiation have not been identified, but some growth factors (members of the TGF beta superfamily) have been demonstrated to be associated with this matrix, and in situ hybridization has demonstrated the presence of transcripts for TGF beta s and BMP-2 in odontoblasts facing differentiating ameloblasts. Moreover, intense expression of receptors for the cytokine interleukin-7 (IL-7) in polarizing ameloblasts has been reported. In this study, isolated E-18 and E-19 mouse molar enamel organs were cultured in vitro in presence of BMP-2, TGF beta-1, IL-7 or synthetic apatite. TGF beta-1 and BMP-2 combined with heparin induced cytodifferentiation of ameloblasts. IL-7 maintained the polarized state of ameloblasts. BMP-2-soaked apatite induced functional differentiation of ameloblasts (secretion of amelogenin). Integrating these data with previous work, a working hypothesis concerning the control of ameloblast terminal differentiation is presented: members of the TGF beta superfamily secreted by odontoblasts might be trapped by predentin components first and then by dentin apatites, and these growth factors might trigger the cytological-functional sequence of ameloblast terminal differentiation.