Association mapping in durum wheat grown across a broad range of water regimes
Association mapping in durum wheat grown across a broad range of water regimes
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DOI：
10.1093/jxb/erq287
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发表时间：
2011-01-01
影响因子：
6.9
通讯作者：
Tuberosa, Roberto
中科院分区：
文献类型：
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作者：
Maccaferri, Marco;Sanguineti, Maria Corinna;Tuberosa, Roberto
利用关联图谱分析了189个优质硬粒小麦材料的干旱适应性状和籽粒产量的遗传基础，这些材料在作物周期（从146到711 mm）和籽粒产量（从9.9到67.3 q ha(-1)） 15个高度不同的环境中进行了评估。对于高遗传性状（如抽穗日期、粒重等），在5个或更多（粒重多达13个）环境中检测到几个显著的实验标记-性状关联，R-2值约为5%至10%。与GY的显著相关性（R-2从2.5到4.2%）主要存在于1个环境（56个标记）中，从2个环境到5个环境（分别从20个标记到3个标记）和1个标记（chr上的Xbarc197）中迅速下降。5A)在六种环境（从低产到高产）中发现显著性。这些结果可能是由于GY的复杂遗传基础及其与环境条件的相互作用。干旱条件下显著影响果龄的标记数量显著减少，表明低水分条件下关联定位鉴定果龄位点的有效性有限，这很可能是因为不同的基因型可以通过不同的形态生理性状和相应的基因网络获得相似的表型。我们的研究证实了以前在双亲本作图群体中描述的物候主要位点的作用，强调了一组新的干旱适应性状位点，并提供了这些位点上的等位基因在广泛的土壤湿度条件下的农艺价值信息。
Association mapping was used to dissect the genetic basis of drought-adaptive traits and grain yield (GY) in a collection of 189 elite durum wheat accessions evaluated in 15 environments highly different for water availability during the crop cycle (from 146 to 711 mm) and GY (from 9.9 to 67.3 q ha(-1)). For highly heritable traits (e.g. heading date, kernel weight, etc.) several significant experiment-wise marker-trait associations were detected across five or more (up to 13 for kernel weight) environments, with R-2 values ranging from ca. 5 to 10%. As to GY, significant associations (R-2 from 2.5 to 4.2%) were mostly detected in one environment only (56 markers), while decreasing rapidly from two to five environments (from 20 to three markers, respectively) and with only one marker (Xbarc197 on chr. 5A) found significant in six environments (ranging from low- to high-yielding). These results are probably due to the complex genetic basis of GY and its interaction with environmental conditions. The number of markers significantly affecting GY decreased considerably under drought conditions, suggesting a limited effectiveness of association mapping to identify loci for GY under low-moisture conditions, most likely because different genotypes can attain similar phenotypes via different morpho-physiological traits and corresponding gene networks. Our study confirmed the role of major loci for phenology previously described in biparental mapping populations, highlighted a novel set of loci for drought-adaptive traits, and provided information on the agronomic value of the alleles at such loci across a broad range of soil moisture conditions.