Expression and function of an early activation marker restricted to human B cells.
Expression and function of an early activation marker restricted to human B cells.
复制标题
仅限于人类 B 细胞的早期激活标记的表达和功能。
DOI：
10.4049/jimmunol.136.11.4019
复制
发表时间：
1986
影响因子：
4.4
通讯作者：
T. Kishimoto
中科院分区：
文献类型：
--
作者：
H. Kikutani;R. Kimura;H. Nakamura;R. Satō;A. Muraguchi;N. Kawamura;R. Hardy;T. Kishimoto
制备了B细胞特异性单克隆抗体（抗Ba）。在双色FACS分析中，抗Ba与从扁桃体获得的IG+或B1+细胞的亚群反应，但不与来自PBL的大多数B1+细胞反应。用TPA或抗mu激活来自PBL的B细胞诱导Ba表达，并加入具有抗mu增强Ba表达的PHA条件上清液。其他B细胞活化剂，如金黄色葡萄球菌科万I（Staph-A）或PWM加T细胞，可以诱导Ba表达。Ba的表达在刺激后6小时观察到，并在72小时达到峰值水平。Ba的表达严格限于B细胞。H-7是一种蛋白激酶C（C-激酶）的特异性抑制剂，对Ba的表达具有剂量依赖性抑制作用，表明Ba的表达依赖于C-激酶。与BSF-1不同，抗Ba抑制抗mu和B-BCGF诱导的B细胞增殖。本研究中呈现的结果表明，B细胞上的Ba抗原可能与T细胞上的Tac抗原相当。
A B cell-specific monoclonal antibody (anti-Ba) was prepared. In two-color FACS analysis the anti-Ba reacted with a subpopulation of Ig+ or B1+ cells obtained from tonsils, but did not react with most B1+ cells derived from PBL. Activation of B cells from PBL with TPA or anti-mu induced Ba expression and the addition of PHA-conditioned supernatant with anti-mu-enhanced Ba expression. Other B cell activators, such as Staphylococcus aureus Cowan I (Staph-A) or PWM plus T cells, could induce Ba expression. Ba expression was observed 6 hr after stimulation and reached a peak level at 72 hr. Ba expression was strictly restricted to B cells. H-7, a specific inhibitor of protein kinase C (C-kinase), displayed a dose-dependent inhibitory effect on Ba expression, showing dependency on C-kinase for Ba expression. Anti-Ba inhibited B cell proliferation induced by anti-mu and B-BCGF distinct from BSF-1. The results presented in this study suggest that the Ba antigen on B cells may be comparable to the Tac antigen on T cells.