Two interleukin 1 genes in the mouse: cloning and expression of the cDNA for murine interleukin 1 beta.
Two interleukin 1 genes in the mouse: cloning and expression of the cDNA for murine interleukin 1 beta.
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小鼠中的两个白细胞介素 1 基因：鼠白细胞介素 1 β cDNA 的克隆和表达。
DOI：
10.4049/jimmunol.137.11.3644
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发表时间：
1986
影响因子：
4.4
通讯作者：
D. Pennica
中科院分区：
文献类型：
--
作者：
P. Gray;D. Glaister;E. Chen;D. Goeddel;D. Pennica
使用人白细胞介素 1 β (IL 1 β) cDNA 探针来鉴定同源鼠 cDNA 克隆。鼠 cDNA 编码 269 个残基的蛋白质，与人 IL 1 β 有 67% 同源性。该鼠类序列经过工程改造，可在哺乳动物细胞中表达，并指导生物活性 IL 1 的合成。这种蛋白质称为鼠类 IL 1 beta，与之前描述的鼠类 IL 1 序列仅 22% 同源。 IL 1 α 和 IL 1 β 均由单个基因编码，但在受刺激的巨噬细胞系中，IL 1 β mRNA 的丰度约为五倍。
A human interleukin 1 beta (IL 1 beta) cDNA probe was utilized to identify a homologous murine cDNA clone. The murine cDNA encodes a 269-residue protein which is 67% homologous to human IL 1 beta. The murine sequence was engineered for expression in mammalian cells and directs the synthesis of biologically active IL 1. This protein, termed murine IL 1 beta, is only 22% homologous with the previously described murine IL 1 sequence. Both IL 1 alpha and IL 1 beta are encoded by single genes, but IL 1 beta mRNA is about fivefold more abundant in a stimulated macrophage cell line.