Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy
Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy
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DOI：
10.1117/1.2210907
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发表时间：
2006-05-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Oh, Jung-Taek;Li, Meng-Lin;Wang, Lihong V.
双波长反射模式光声显微镜用于非侵入性地获得裸鼠体内皮下黑色素瘤及其周围脉管系统的三维（3-D）图像。血液和黑色素性黑色素瘤的吸收系数在这两个光学波长（764和584 nm）处相对于彼此变化很大。在体内使用高分辨率和高对比度的光声成像，使用近红外（764 nm）光源，对皮肤内的3-D黑色素分布进行成像，并测量黑色素瘤的最大厚度（类似于0.5 mm）。可见光（584 nm）也可对黑色素瘤周围的血管系统成像，并发现肿瘤供血血管，该技术可用于黑色素瘤的诊断、预后和治疗计划。(c)2006年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
Dual-wavelength reflection-mode photoacoustic microscopy is used to noninvasively obtain three-dimensional (3-D) images of subcutaneous melanomas and their surrounding vasculature in nude mice in vivo. The absorption coefficients of blood and melanin-pigmented melanomas vary greatly relative to each other at these two optical wavelengths (764 and 584 nm). Using high-resolution and high-contrast photoacoustic imaging in vivo with a near-infrared (764nm ) light source, the 3-D melanin distribution inside the skin is imaged, and the maximum thickness of the melanoma (similar to 0.5 mm) is measured. The vascular system surrounding the melanoma is also imaged with visible light (584 nm) and the tumor-feeding vessels found. This technique can potentially be used for melanoma diagnosis, prognosis, and treatment planning. (c) 2006 Society of Photo-Optical Instrumentation Engineers.