A field deployable method for a rapid screening analysis of inorganic arsenic in seaweed.
A field deployable method for a rapid screening analysis of inorganic arsenic in seaweed.
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DOI：
10.1007/s00604-017-2151-1
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Feldmann J
中科院分区：
文献类型：
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作者：
Bralatei E;Nekrosiute K;Ronan J;Raab A;McGovern E;Stengel DB;Krupp EM;Feldmann J
采用一种新开发的现场部署方法，借助水中砷现场检测试剂盒，测定了13个商店购买的食用海藻样品和34个干海带样品中的无机砷(IAS)。并与高效液相色谱-电感耦合等离子体质谱(HPLC-ICPMS)进行形态分析的结果进行比较。该法包括一种简单的萃取方法，用稀释的HNO3定量提取IAS，而不将有机砷分解为IAS，然后IAS选择性挥发为砷(AsH3)，然后在浸泡在溴化汞(HgBr2)溶液中的滤纸上进行化学捕集。方法对样品进行优化，结果表明，FDM法的IAS回收率为80-94%，而二甲基砷酸(DMA)形式的有机砷对IAS浓度无基质影响。该方法重现性好，平均误差为±19%，经高效液相-电感耦合等离子体质谱验证，结果与形态分析结果一致(斜率=1.03，R2=0.70)，无偏倚。该法可在1小时内完成，观察到的定量限约为0.05mgkg−1(干重)。该方法适用于海藻收获前的IAS现场监测，可作为海藻IAS的筛选方法。通过使用现场可部署的砷试剂盒，可以在一小时内无偏倚地筛选海藻的无机砷浓度。并将其准确度和精密度与高效液相色谱-离子色谱质谱仪进行了比较。本文的在线版本(doi：10.1007/s00604-0172151-1)包含补充材料，授权用户可以使用。
Inorganic arsenic (iAs) in 13 store-bought edible seaweed samples and 34 dried kelp (Laminaria digitata) samples was determined by a newly developed, field-deployable method (FDM) with the aid of a field test kit for arsenic in water. Results from the FDM were compared to results from speciation analysis achieved by using high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The FDM consisted of a simple extraction method using diluted HNO3 to quantitatively extract iAs without decomposing the organoarsenicals to iAs followed by the selective volatilisation of iAs as arsine (AsH3) and subsequent chemo-trapping on a filter paper soaked in mercury bromide (HgBr2) solution. Method optimization with a sub-set of samples showed 80–94% iAs recovery with the FDM with no matrix effect from organo-arsenic species in the form of dimethylarsinic acid (DMA) on the iAs concentration. The method displayed good reproducibility with an average error of ±19% and validation by HPLC-ICP-MS showed that the results from the FDM were comparable (slope = 1.03, R2 = 0.70) to those from speciation analysis with no bias. The FDM can be conducted within an hour and the observed limit of quantification was around 0.05 mg kg−1 (dry weight). This method is well suited for on-site monitoring of iAs in seaweed before it is harvested and can thus be recommended for use as a screening method for iAs in seaweed. Screening seaweed for their inorganic arsenic concentration within one hour without bias has been made possible in the field by using a field deployable arsenic kit. Its accuracy and precision was compared to HPLC-ICPMS. The online version of this article (doi:10.1007/s00604-017-2151-1) contains supplementary material, which is available to authorized users.