Identification and characterization of homologues of the Exocyst component Sec10p
Identification and characterization of homologues of the Exocyst component Sec10p
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DOI：
10.1016/s0014-5793(97)00109-9
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发表时间：
1997-03-10
期刊：
影响因子：
3.5
通讯作者：
Novick, P
中科院分区：
文献类型：
--
作者：
Guo, W;Roth, D;Novick, P
SEC10基因产物是酿酒酵母胞吐作用所必需的胞囊复合体的一员，我们报道了人Sec10p(hSec10p；GenBank登录号U85946)的克隆和鉴定。HSec10p是一个77 kDa的蛋白，与酵母Sec10p的氨基酸同源性为23%，与数据库中发现的线虫蛋白的氨基酸同源性为37%。Northern和Western印迹分析表明，hSec10具有广泛的组织分布，免疫荧光染色显示，hSec10p和哺乳动物Sec8p共转染COS细胞后，这两种蛋白在细胞内的分布相同，包括在外周细胞质中的定位。这些数据表明，hSec10p是哺乳动物中对后高尔基体运输至关重要的酵母外囊复合体的一种成分。(C)1997年欧洲生化学会联合会。
The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae, We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% identity to a C. elegans protein found in the database. Northern and Western blot analyses indicate that hSec10 has a broad tissue distribution, Immunofluorescence staining of COS cells cotransfected with hSec10p and a mammalian Sec8p demonstrates that these two proteins have an identical distribution in the cell including a localization in the peripheral cytoplasm, These data suggest that hSec10p is a component of the mammalian counterpart of the yeast Exocyst complex essential for post-Golgi traffic. (C) 1997 Federation of European Biochemical Societies.